5x ELISA Coating Buffer | Accessory Reagent

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  • 5x ELISA Coating Buffer
  • 5x ELISA Coating Buffer
  • 5x ELISA Coating Buffer
(Rated 0.0/5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Format Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF030AE100 ml
    BUF030A
    BUF030CE1000 ml
    BUF030C
    BUF030BE500 ml
    BUF030B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      BUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

      BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.
    • Product Form
      5 x concentrate - liquid
      5 x concentrate - liquid
      5 x concentrate - liquid
    • Preservative Stabilisers
      0.5%Sodium Azide
      0.5%Sodium Azide
      0.5%Sodium Azide
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
    • Shelf Life
      24 months from date of despatch.
      24 months from date of despatch.
      24 months from date of despatch.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNo
      ELISA
    • Application NameYesNo
      ELISA
    • Application NameYesNo
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.
      Please view the datasheet pdf for suggested dilutions.
    • Instructions For Use
      1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

      2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

      3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
    • Instructions For Use
      1. Dilute the required volume of BUF030C 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

      2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

      3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.
    • Instructions For Use
      1. Dilute the required volume of BUF030B 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

      2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

      3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

    Additional 5x ELISA Coating Buffer Formats

    FormatsApplicationsSizes available
    5x ELISA Coating Buffer : 5 X ConcentrateE100 ml | 1000 ml | 500 ml
    • Copyright © 2014 Bio-Rad AbD Serotec

    Recommended Secondary Antibody

    Recommended Negative Isotype Control

    Useful Reagents

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      10x ELISA Wash BufferBUF031A100 mlE
      BUF031A
      ELISA BSA BlockBUF032A100 mlE
      BUF032A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      10x ELISA Wash BufferBUF031A100 mlE
      BUF031A
      ELISA BSA BlockBUF032A100 mlE
      BUF032A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      10x ELISA Wash BufferBUF031A100 mlE
      BUF031A
      ELISA BSA BlockBUF032A100 mlE
      BUF032A

      Recommended Positive Controls

      Histology Controls

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