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5x ELISA Coating Buffer

  • Product Code
  • Description
    5x ELISA Coating Buffer
  • Product Type
    Accessory Reagent
  • Format
    5 X Concentrate
  • Applications
  • Pack Size
    100 ml
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
More Information

5x ELISA Coating Buffer Product Summary

Intended Use

BUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.

Product Form

5 x concentrate - liquid

Preservative Stabilisers

0.5%Sodium Azide

Buffer Solution

Phosphate buffered saline


Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.

Shelf Life

24 months from date of despatch.

5x ELISA Coating Buffer Additional Formats

  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    5 X ConcentrateBUF030C1000 mlE
    5 X ConcentrateBUF030B500 mlE

5x ELISA Coating Buffer Related Reagents

Useful Reagents

FormatProduct CodePack SizeApplicationsList PriceQuantity
ConcentrateBUF031A100 mlE
Ready To UseBUF032A100 mlE

More Reagents With This Specificity

5x ELISA Coating Buffer More Images

5x ELISA Coating Buffer Application Notes


This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit
Application NameYesNoNot DeterminedSuggested Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.

Instructions For Use

1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

5x ELISA Coating Buffer References

5x ELISA Coating Buffer More Information

Health And Safety Information

Material Safety Datasheet Documentation #10383 available at:


For research purposes only
Copyright © 2013 Bio-Rad AbD Serotec

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