List Price Quantity
BUF030A
TBC
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5x ELISA Coating Buffer

  • Product Code
    BUF030A
  • Description
    5x ELISA Coating Buffer
  • Product Type
    Accessory Reagent
  • Format
    5 X Concentrate
  • Applications
    E
  • Pack Size
    100 ml
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

5x ELISA Coating Buffer Product Summary

Intended Use

BUF030A is a specialist coating buffer for use in all ELISA formats. Designed to stabilize the tertiary structure of the antibody or antigen and maximize adsorption onto polystyrene plates. It is especially effective in sandwich ELISA, preserving the antigen recognition regions of the coating antibody, allowing greater binding reactivity.

BUF030A contains an antimicrobial agent allowing coating to be performed at room temperature. Dried plates can be kept at 4°C for long term storage.

Product Form

5 x concentrate - liquid

Preservative Stabilisers

0.5%Sodium Azide

Buffer Solution

Phosphate buffered saline

Storage

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.

Shelf Life

24 months from date of despatch.

5x ELISA Coating Buffer Additional Formats

  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    5 X ConcentrateBUF030C1000 mlE
    BUF030C
    5 X ConcentrateBUF030B500 mlE
    BUF030B

5x ELISA Coating Buffer Related Reagents

Useful Reagents

FormatProduct CodePack SizeApplicationsList PriceQuantity
ConcentrateBUF031A100 mlE
BUF031A
Ready To UseBUF032A100 mlE
BUF032A

More Reagents With This Specificity

5x ELISA Coating Buffer More Images

5x ELISA Coating Buffer Application Notes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
Application NameYesNoNot DeterminedSuggested Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only.

Instructions For Use

1. Dilute the required volume of BUF030A 1:5 with deionised water and mix for 5-15 minutes to prepare the working solution.

2. Add antibody or antigen to the working solution and mix for 5-15 minutes.

3. Coat plate (suggested volume 100-200µl per well) and incubate at room temperature for 6-24 hours, protected from light and covered to avoid evaporation.

5x ELISA Coating Buffer References

5x ELISA Coating Buffer More Information

Health And Safety Information

Material Safety Datasheet Documentation #10383 available at:
http://www.abdserotec.com/uploads/MSDS/10383.pdf

Regulatory

For research purposes only
Copyright © 2013 Bio-Rad AbD Serotec

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