Alexa Fluor 488 Conjugated Antibodies

Alexa Fluor® 488 dyes, part of our Alexa Fluor fluorescent conjugates range, give you outstanding results in flow cytometry and immunofluorescence / confocal microscopy.

Fig.1. Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 (MCA497PE), red and Rat anti Mouse CD45 (MCA1031A488), green; nuclei are stained with DAPI, blue.

Fig.1. Immunofluorescence image of mouse small intestine stained with Rat Anti-Mouse F4/80 (MCA497PE), red and Rat Anti-Mouse CD45 (MCA1031A488), green; nuclei are stained with DAPI, blue.

The image highlights macrophages within the lamina propria.

Alexa Fluor 488 dyes, sometimes referred to as AF488, are:

  • The finest green fluorophore available
  • Excited by an argon-ion laser
  • Usually detected in the same fluorescence channel as FITC
  • Alternative for fluorescein conjugates (FITC, FAM) and Cy2
  • Brighter and more photostable than fluorescein

Technical Features of Alexa Fluor 488 Dye

Fluorochrome Maximal absorbance (nm) Maximal emission (nm) Flow cytometry Confocal microscopy Color
Alexa Fluor 488 495 519 Yes Yes Green

Antibodies Conjugated to Alexa Fluor 488

Bio-Rad offers primary antibodies conjugated to Alexa Fluor 488 across human, mouse, and rat catalog ranges.

Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.

AF488 Conjugated Antibodies

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code Validation Types

    Fluorophore Dyes

    Other dye formats available in our flow cytometry range include:

    More details on emission and excitation values can be found on our Fluorophores page.