Product Images

  • Dot-plot showing Ramos cells staining with Annexin V:FITC (ANNEX100F) versus Propidium Iodide resulting in three distinct populations

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List Price Quantity
ANNEX100F
TBC

Annexin V Kit

  • Product Code
    ANNEX100F
  • Description
    Annexin V:FITC Assay Kit
  • Product Type
    Kits
  • Format
    FITC
  • Applications
    F
  • Pack Size
    100 Tests
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Annexin V Kit Product Summary

Product Information

This test employs the property of Annexin V:FITC to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.

The conjugation protocol used to prepare Annexin V:FITC has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.

Preservative Stabilisers

0.02%Sodium Azide (NaN3)
1%Bovine Serum Albumin

Reagents In The Kit

Annexin V:FITC1 x 0.5ml vial
Propidium Iodide1 x 1.6ml vial at 20ug/ml
Binding buffer1 x 50ml vial at x 4 concentrate

Storage

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Annexin V Kit Additional Formats

  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    BiotinANNEX100B100 TestsF
    ANNEX100B

Annexin V Kit Related Reagents

Useful Reagents

FormatProduct CodePack SizeApplicationsList PriceQuantity
BiotinANNEX100B100 TestsF
ANNEX100B
BiotinANNEX300B300 TestsF
ANNEX300B
FITCANNEX300F300 TestsF
ANNEX300F

More Reagents With This Specificity

Annexin V Kit More Images

    • Product Images

    • Dot-plot showing Ramos cells staining with Annexin V:FITC (ANNEX100F) versus Propidium Iodide resulting in three distinct populations
  • Application NameReference Images
    Flow Cytometry

Annexin V Kit Application Notes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
Application NameYesNoNot DeterminedSuggested Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use

1) Dilute the binding buffer 1:4 in distilled water (50ml binding buffer +150ml distilled water)

2) Wash cells in PBS by gentle shaking or by pipetting up and down.

3) Resuspend cells in 200ul pre-diluted binding buffer, adjusting to a cell density of 2-5 x 105 cells/ml.

4) Add 5ul Annexin V:FITC to 195ul of the cell suspension prepared in step 3.

5) Mix and incubate for 10 minutes in the dark, at room temperature.

6) Wash cells in 200ul of pre-diluted binding buffer.

7) Resuspend cells in 190ul pre-diluted binding buffer.

8) Add 10ul of the Propidium Iodide solution.

9) Analyse by flow cytometry.

The flow cytometer is preferably set such that the Mean Fluorescence Intensity of the Annexin V negative population is between 1 and 10. Optimal parameter settings can be found using a positive control. For a positive control, incubate the cells with 3% formaldehyde in buffer during 30 minutes on ice. Wash away the formaldehyde and suspend the cells in cold binding buffer at 2-5 x 105 cells/ml. Proceed with step 2 as described above.

Annexin V Kit References

References

1. Lu, K.H. et al. (2010) In Vitro and In Vivo Apoptosis-Inducing Antileukemic Effects of Mucuna macrocarpa Stem Extract on HL-60 Human Leukemia Cells.
Integr Cancer Ther. 9: 298-308.
2. Yen, J. et al. (2010) Glycine tomentella Hayata inhibits IL-1ß and IL-6 production, inhibits MMP-9 activity, and enhances RAW264.7 macrophage clearance of apoptotic cells.
J. Biomed. Sci. 17: 83-91.
3. Chen, C.W. et al. (2010) The signals of FGFs on the neurogenesis of embryonic stem cells.
J Biomed Sci.17:33.
4. Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.
Immunology. 132: 256-65.
5. Lu, K.H. et al. (2012) Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism.
Evid Based Complement Alternat Med. 2012:921430.

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