This test employs the property of Annexin V:FITC to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.
The conjugation protocol used to prepare Annexin V:FITC has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.
|0.02%||Sodium Azide (NaN3)|
|1%||Bovine Serum Albumin|
Reagents In The Kit
|Annexin V:FITC||1 x 0.5ml vial|
|Propidium Iodide||1 x 1.6ml vial at 20ug/ml||Binding buffer||1 x 50ml vial at x 4 concentrate|
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch.