ELISA/Western Blotting Blocking Agents and Accessory Buffers

Improve Your Results by Eliminating Backgrounds

• Easy to use
• Reduce non-specific binding
• Reliable consistent formulation
• Choice of blocking strengths

Block Ace

Superior to 1% BSA, BLOCK ACE is a high-performance blocking reagent for ELISA assays and Western blotting. In comparison to BSA, BLOCK ACE provides reduced backgrounds and sharper standard curves. It can also be used as a wash buffer, and for diluting antibodies.

BSA Block

Minimize non-specific binding in direct and indirect ELISAs using BSA BLOCK. Based on BSA, this buffer has a proprietary protein stabilizer that creates a long-term stable environment for coating antigens or capture antibodies.

ELISA Synblock

For maximum blocking strength use SYNBLOCK. The inert nature of this novel and unique blocking agent eliminates background interference associated with sandwich ELISA assays.

ELISA Ultrablock

Enables the detection of tiny amounts of specific antigen. Due to the small size of the blocking agent (based on fish proteins), ULTRABLOCK can coat non-specific binding sites that are sterically inaccessible to traditional blockers. By virtually eliminating the background it vastly enhances the sensitivity of your assay.

ELISA Neptune Buffer

Avoid cross-reactivity with mammalian proteins with this ideal companion to ELISA ULTRABLOCK. This buffer is also based on fish proteins and reduces non-specific binding between the sample matrix and the protein adsorbed to the ELISA plate.

ELISA General Buffer

Improves standard curves and reduces false positives by stabilizing the non-specific background generated by the sample matrix and diluent. The buffer contains goat serum proteins and is recommended for plasma, serum or cell culture samples.

ELISA IgM Reducing Buffer

Diminishes background noise in problematic samples such as plasma, serum or cell culture. It contains a chelator to help prevent interference from complement and thrombin, and agents to reduce IgM-related non-specific binding between serum/plasma proteins and the plate surface.

ELISA Wash Buffer

Ready to use alternative to traditional PBS and TWEEN buffers. The optimized formulation of pH stabilisers, salts and detergents increases the efficiency of washing stages during ELISAs. This leads to a reduction in background noise and an increased specific signal.

ELISA Diluent-Rabbit Polyclonal

For outstanding results with HRP conjugates use this buffer. It has been specially designed to stabilize HRP in reconstituted antibodies and to prevent non-specific HRP staining.

ELISA Coating Buffer

Stabilizes and maximizes protein adsorption onto polystyrene plates. The antigenic regions of the coating protein are preserved allowing greater binding reactivity and an enhanced specific signal.

HiSpec Assay Diluent

Reduces cross reactivity, non-specific binding and matrix effects in immunoassays, including immunohistochemistry. HiSpec is designed to reduce interference in immunoassays and optimizes results.

Standard Block

High Performance Block

Problematic Samples (e.g. serum, cell culture or mammalian proteins)

Rabbit HRP Conjugates

All ELISA’s