Apoptosis Kits: Caspase, Annexin V, Cathepsin, Mitochondrial Membrane, Apo-BrdU™ TUNEL Assay

Choose from a wide range of apoptosis kits including:

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Annexin V Kits

AbD Serotec offers a quick and easy method for detecting apoptotic cells by flow cytometry with the Annexin V apoptosis kits.

Image: Dot-plot showing Ramos Cells staining with Annexin V-FITC versus Propidium lodide.

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Caspase FLICA™ Kits

Our wide range of Caspase FLICA™ apoptosis detection kits, allow analysis of active caspases in whole, living cells. The methodology of these caspase assay kits is based on a unique cell-permeable and non-cytotoxic reagent.

Image: Results using Poly caspase FLICA™ kits (Product Code ICT091 & ICT092) analyzed by fluorescence microscopy show suspension cells labeled with FAM-VAD-FMK, and Hoechst 33342 stain. Both cells are apoptotic and dying - detected by (green) caspase activity and (blue) nuclear Hoechst staining. The left cell is much brighter green than the right cell, indicating that the left cell has more active caspases.

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Mitochondrial Membrane Potential (MitoPT™) Kits

The JC-1, TMRE and TMRM MitoPT™ Kits allow clear differentiation between non-apoptotic and apoptotic cells through the simple and reproducible detection of the mitochondrial membrane permeability transition event.

Image: Jurkat cells stained with MitoPT™ JC-1 (Product Code ICT943 & ICT944) and viewed through a fluorescence microscope. Non-apoptotic cells exhibit red stained mitochondria (2 left hand cells). Apoptotic cells at varying stages of mitochondrial membrane permeability appear green (3 right hand cells).

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Apo-BrdU™ TUNEL Assay KitsApo-BrdU™ TUNEL Assay Kits

AbD Serotec’s Apo-BrdU™ TUNEL assay is based on the addition of labeled nucleotides to the free 3’-hydroxyl termini of double or single stranded DNA fragments. This method makes it easy to identify apoptotic cells since the DNA of healthy cells remains intact.

The TUNEL assay labels DNA fragments with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP) through a reaction catalyzed by terminal deoxynucleotidyl transferase (TdT).

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Magic Red™ Cathepsin Kits

We offer Magic Red™ Kits for analysis of active cathepsins in whole, living cells, utilizing a substrate based assay. These kits enable the detection of active cathepsins contained in lysosomes which are involved in the autophagy pathway, phagocytosis and when released into the cytoplasm can regulate both the apoptosis extrinsic and intrinsic pathways.

Image: Intracellular cathespin L activity detected in THP-1 cells using Magic Red™ Cathepsin L Kit (Products Code ICT941 / 942)

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Apo-BrdU™ is a registered trademark of Phoenix Flow Systems USA.
FLICA™, Magic Red™ and Mito PT™ are trademarks of Immunochemistry Technologies Inc. USA.