Western Blotting

AbD Serotec is one of the world’s leading manufacturers of primary and secondary antibodies for Western blot anaylsis. In addition, we offer ISO-certified pre-made buffers to simplify immunostaining and make results more consistent from blot to blot. Our 100% Satisfaction Guarantee is backed by our quality, which makes ordering from AbD Serotec totally risk free!

• Buffers & Accessories
• Western Blotting Loading Control Antibodies
• Secondaries

Buffers & Accessories

Our range of specialized reagents are designed to significantly improve results in Western blotting:

Block Ace This high-performance blocking reagent for Western blotting is superior to 1% BSA. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves. It can also be used as a wash buffer, and for diluting antibodies.

HiSpec This assay diluent works to significantly reduce cross-reactivity and non-specific binding in Western blotting, greatly improving your results. HiSpec assay diluent is designed to be used instead of sample buffer or antibody dilution buffer within the Western blotting protocol.

AbGuard® HRP Stabilizer Is ideal for use as a diluent for peroxidase conjugated antibodies and other peroxidase conjugated proteins. It provides superior stabilization of HRP-conjugates at low and high dilutions, preventing the loss of peroxidise activity.

Western Blotting Loading Control Antibodies

AbD Serotec offers a range of antibodies to popular loading controls suitable for Western blotting. Our loading control antibodies are offered in a variety of different formats ideal for saving time with direct staining techniques and for use in infrared imaging systems e.g. LI-COR Odyssey®.

Loading Control Specificity

• Beta Actin
• Cyclophilin A
• GAPDH
• Tubulin Alpha

Secondaries

Horseradish-peroxidase conjugated antibodies are preferentially used whenever an indirect method is used for detection on Western Blots. AbD Serotec supplies a range of species specific antibodies recognizing immunoglobulin or any of its isotypes. Monoclonal and polyclonal secondary antibodies are available, as a whole IgG or a F(ab’)2 fragment. Performance is further enhanced by affinity purification for all STAR reagents and many anti-mouse, -rat, and -hamster secondary antibodies are multi-species adsorbed.

LI-COR Odyssey® is a registered trademark of LI-COR Biosciences, Inc. USA.

AbGuard® is a registered trademark of MorphoSys UK Ltd