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Datasheet: 730001S

Description: PROTEIN A
Name: PROTEIN A
Applications: E
Format: Purified
Product Type: Recombinant Protein
Quantity: 1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
ELISA   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Bacterial
Product Form
Purified protein - lyophilised
Reconstitution
Reconstitute with 1.0 ml sterile PBS
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Source
Staphylococcus aureus
Buffer Solution
N/A
Preservative Stabilisers
None present
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml after reconstitution
Description
Protein A is a 40-60 kDa MSCRAMM (microbial surface components recognizing adhesive matrix molecules) surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR.

Protein A binds the Fc region of various mammalian IgG subclasses including:

Human IgG1, IgG2, IgG4
Mouse IgG2a, IgG2b, IgG3
Rat IgG1, IgG2c
Guinea pig IgG1, IgG2
Rabbit IgG
Dog IgGA, IgGB, IgGC, IgGD
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
12 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request)

Other formats and applications

This reagent is also available in a HRP format and is useful for Immunohistology - Frozen

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M102012:081030'