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Datasheet: AHP1053
Description: RABBIT ANTI 14-3-3
Specificity: 14-3-3
Applications: IP, P, WB
Format: Serum
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.1 ml
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
Immunoprecipitation   
Western Blotting  1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Sheep
Species Cross Reactivity
Reacts with: Human, Bovine, Rat, Mouse, Amphibia, Chicken, Fungal, Plants, Reptile
Based on sequence similarity, is expected to react with:Mammals
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to sheep 14-3-3 protein were raised by repeated immunisations of rabbit with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Mixture of synthetic peptides corresponding to all isoforms of sheep 14-3-3.
External Database Links
Click for detail UniProt:
P682511433B_SHEEPRelated reagents
P622621433E_SHEEPRelated reagents
P293611433Z_SHEEPRelated reagents
O776421433S_SHEEPRelated reagents
P682531433G_SHEEPRelated reagents
Click for detail Entrez Gene:
443032SFNRelated reagents
Click for detail GO:
GO:0003779actin bindingRelated reagents
GO:0005159insulin-like growth factor receptor bindingRelated reagents
GO:0045664regulation of neuron differentiationRelated reagents
GO:0048167regulation of synaptic plasticityRelated reagents
Specificity
AHP1053 recognises all isoforms of the 14-3-3 protein family. The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
Immunohistology
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Western Blotting
AHP1053 detects a band of approximately 30kDa in HEK293 cell lysates.
References
1. Aitken, A et al. (2006) 14-3-3 proteins: A historic overview. Seminars in cancer biology 16:162-172.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

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Sheep Anti Rabbit IgG (STAR36...) DyLight®488, DyLight®549, DyLight®649, DyLight®800
Sheep Anti Rabbit IgG (STAR35...) RPE
Sheep Anti Rabbit IgG (STAR34...) FITC
Goat Anti Rabbit IgG (Fc) (STAR121...) FITC, HRP
Goat Anti Rabbit IgG (H/L) (STAR124...) HRP
Sheep Anti Rabbit IgG (2AB02...) Biotin
Sheep Anti Rabbit IgG (STAR54...) HRP

Recommended Useful Reagents

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M151248:100317'


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Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
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Printed on 04 Sep 2010