Browsing Modes for Business Users

TMB Signal+

Product Code Applications Pack Size List Price Your Price Qty
BUF054B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml loader
List Price Your Price
loader
BUF054A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml loader
List Price Your Price
loader

Intended Use
BUF054B: BUF054B is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP).

BUF054B contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB SIGNAL+ has been optimised to enable increased sensitivity whilst maintaining increased stability. BUF054B provides minimal background and rapid development.

BUF054B produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
BUF054A: BUF054A is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP).

BUF054A contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB SIGNAL+ has been optimised to enable increased sensitivity whilst maintaining increased stability. BUF054A provides minimal background and rapid development.

BUF054A produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Product Form
Ready to use TMB solution - liquid
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product is photosensitive and should be protected from light.

Avoid exposure to heat and contamination with metal ions or peroxidase.

Store in bottles made of High Density Polyethylene (HDPE).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Pour the desired amount of BUF054B into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well.

2. Add 0.1ml of BUF054B per microtiter well.

3. Allow development of BUF054B. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF054B is not recommended).
1. Pour the desired amount of BUF054A into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well.

2. Add 0.1ml of BUF054A per microtiter well.

3. Allow development of BUF054A. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil.

4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour.

5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of BUF054A is not recommended).

BUF054B

154850 157423 162400 180116

BUF054A

152093 155152 156780 160071 163243 165352

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with TMB specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note