This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Human

Peripheral Blood Leukocyte (PBL's) tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. **

None present.

Total protein concentration 2mg/ml

This lysate is recommended for use in Western blotting. 10ug to 20ug per lane is recommended for mini gel.

Store at 2-8^oC for continuous use. For extended storage, freeze working aliquots at -70^oC. Repeated freezing and thawing is not recommended.

6 months from date of despatch

(A full Health and Safety assessment is available upon request)