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Datasheet: MCA2334
Description: MOUSE ANTI BOVINE TNF ALPHA
Specificity: TNF ALPHA
Applications: E, F*
Format: Purified
Product Type: Monoclonal Antibody
Clone: CC327
Isotype: IgG2b
Quantity: 0.5 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry(1)   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1) Special conditions apply - see [*]
Target Species
Bovine
Species Cross Reactivity
Does not react with:Sheep
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Recombinant bovine TNF alpha
External Database Links
Click for detail UniProt:
Q06599TNFA_BOVINRelated reagents
Click for detail Entrez Gene:
280943TNFRelated reagents
Synonyms
TNFA, TNFSF2
Specificity
MCA2334 recognises bovine TNF alpha, a 17.5kDa cytokine, expressed by many different stimulated cell types including monocytes, macrophages, endothelial cells, fibroblasts and both T and B-lymphocytes.

The production of TNF alpha is induced by a variety of factors, dependant upon cell type and includes bacterial toxins, IL-1, PDGF, IFN-beta, NGF, Oncostatin M and viral infections. The presence of TNF alpha is responsible for diverse immunomodulatory, anti-tumour and toxic effects and under certain conditions is also capable of self-stimulation and inhibition.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
*Membrane permeabilisation is required for this application. Serotec recommends the use of LeucopermTM (Fix & PermTM) Product code: BUF09 for this purpose.
Method sheets are available on request.
ELISA
This reagent may be used as a capture antibody in sandwich ELISA assays for bovine TNF alpha in combination with MCA2335B as detection reagent.
References
1. Hope, J.C. et al. (2003) Maturation of bovine dendritic cells by lipopeptides. Vet. Immunol. Immunopathol. 95: 21-31.
2. Whelan A.O. et al. (2003) Modulation of the bovine delayed-type hypersensitivity responses to defined mycobacterial antigens by a synthetic bacterial lipopeptide. Infect. Immun. 71: 6420-6425.
3. Guergnon, J. et al. (2003) A tumour necrosis factor alpha autocrine loop contributes to proliferation and nuclear factor-kappaB activation of Theileria parva-transformed B cells. Cell. Microbiol. 5: 709-716.
4. Kwong, L.S. et al. (2010) Production and characterization of two monoclonal antibodies to bovine tumour necrosis factor alpha (TNF-alpha) and their cross-reactivity with ovine TNF-alpha. Vet Immunol Immunopathol. 135: 320-4.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

Other formats and applications

This reagent is also available in a ALEXA FLUOR® 488 format

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M157690:100614'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 29 Jul 2010