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Datasheet: MCA2671GA
Description: MOUSE ANTI HUMAN CD243
Specificity: CD243
Other names: MULTIDRUG RESISTANCE PROTEIN 1
Applications: C, F, IF, IP, P*
Format: Purified
Product Type: Monoclonal Antibody
Clone: UIC2
Isotype: IgG2a
Quantity: 0.1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/50 - 1/200
Immunohistology - Frozen   
Immunohistology - Paraffin(1)   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Immunofluorescence   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1)Special conditions apply - see *
Target Species
Human
Species Cross Reactivity
Reacts with: Primate
Does not react with:Mouse, Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Immunogen
Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
External Database Links
Click for detail UniProt:
P08183MDR1_HUMANRelated reagents
Click for detail Entrez Gene:
5243ABCB1Related reagents
Click for detail GO:
GO:0005515protein bindingRelated reagents
GO:0005524ATP bindingRelated reagents
GO:0005624membrane fractionRelated reagents
GO:0006810transportRelated reagents
GO:0009986cell surfaceRelated reagents
GO:0016021integral to membraneRelated reagents
GO:0042493response to drugRelated reagents
GO:0042626ATPase activity, coupled to transmembrane movement of substancesRelated reagents
Synonyms
MDR1, PGY1
Specificity
MCA2671GA specifically recognises an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds.

CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.

Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell (2). Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase.

AbD Serotec recommend the use of (MCA2671EL ) for use in functional studies.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Method sheets are available on request.
Immunohistology
*This product may benefit from antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose (3-4).
Histology Positive Control Tissue
Human kidney.
References
1. Mechetner, E.B and Roninson, I.B. (1992) Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. P.N.A.S. 89: 5824-5828.
2. Park, S.W. et al. (2003) Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay. Cytometry Part A. 53A: 67-78.
3. Koziolek, M.J. et al. (2001) Expression of multidrug resistance P-glycoprotein in kidney allografts from cyclosporine A-treated patients. Kid. Int. 60: 156-166.
4. Beck, W.T. et al. (1996) Methods to detect P-glycoprotein-associated multidrug resistance in patients’ tumours: Consensus recommendations. Cancer. Res. 56: 3010-3020.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

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Recommended Negative Controls

Other formats and applications

This reagent is also available in a ALEXA FLUOR® 488, ALEXA FLUOR® 647, Biotin, FITC, Low Endotoxin, RPE format and is useful for Functional Assays

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M138635:090825'


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Email: tech.uk@abdserotec.com
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Printed on 31 Jul 2010