This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Human

Purified IgG - liquid

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Phosphate buffered saline pH7.4

0.09%

Sodium Azide (NaN3)

IgG concentration 1.0mg/ml

Human HPB acute lymphoid leukaemia cell line.

CMRF35H

MCA2704GA specifically recognises human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), a 60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells.

Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul. Method sheets are available on request

MCA2704GA detects a band of approximately 60kDa in HPB cell lysates under non-reducing conditions.

1. Bachelet, I. et al. (2005) The inhibitory receptor IRp60 (CD300a) is expressed and functional on human mast cells. J. Immunol. 175: 7989-7995.

Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

18 months from date of despatch.

(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.