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Datasheet: MCA2838
Description: MOUSE ANTI CHICKEN CD86
Specificity: CD86
Applications: F
Format: Purified
Product Type: Monoclonal Antibody
Clone: IAH:F853:AG2
Isotype: IgG1
Quantity: 0.25 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/40
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
SwissProt ID
Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Plasmid DNA encoding chicken CD86 followed by CD86 Ig-fusion protein.
External Database Links
Specificity
MCA2838 recognises a chicken orthologue of mamallian CD86, an intergral membrane protein, which is a member of the immunoglobulin superfamily. In mammals, CD86 is expressed by mature antigen-presenting cells, including activated B cells, activated T-cells, macrophages and dendritic cells. Mammalian CD86 functions as a costimulatory molecule of T cell through ligation of CD28 and CD152. This antibody recognises the molecule expressed on the surface of COS-7 cells transfected with the cDNA, accession number AM050135 in a mammalian expression vector. It detects a small population of chicken splenocytes stimulated with CD40 ligand.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Method sheets are available on request.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Acknowledgements
This reagent was produced as part of the BBSRC/SEERAD Immunological Toolbox (grant numbers BBS/B/00255, MRI/094/04).
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

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For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M138786:090825'


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Printed on 14 Mar 2010