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Datasheet: MCA3912Z
Description: MOUSE ANTI HUMAN CD322:Azide Free
Specificity: CD322
Applications: P*, WB
Format: Azide Free
Product Type: Monoclonal Antibody
Clone: 1G4
Isotype: IgG1
Quantity: 0.1 mg
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Western blot analysis of CD322 expression in LNCaP using Mouse anti Human CD322:Azide Free (MCA3912Z)
Western blot analysis of CD322 expression in LNCaP using Mouse anti Human CD322:Azide Free (MCA3912Z)
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Immunohistology - Paraffin(1)  0.1 - 10 ug/ml
Western Blotting  0.1 - 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1)Special conditions apply – see(*)
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline pH7.2
Preservative Stabilisers
None present
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Immunogen
Recombinant protein corresponding to aa 29 - 299 of human JAM2
External Database Links
Click for detail UniProt:
P57087JAM2_HUMANRelated reagents
Click for detail Entrez Gene:
58494JAM2Related reagents
Click for detail GO:
GO:0005887integral to plasma membraneRelated reagents
GO:0016337cell-cell adhesionRelated reagents
Synonyms
C21ORF43, VEJAM
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Specificity
MCA3912Z is specific for human CD322.
Immunohistology
*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request)
Image

Western blot analysis of CD322 expression in LNCaP using Mouse anti Human CD322:Azide Free (MCA3912Z)

Staining of paraffin embedded human diffuse large B-cell lymphoma with Mouse anti Human CD322:Azide Free (MCA3912Z)

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For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M118825:090408'


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Printed on 03 Sep 2010