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Datasheet: MCA4622GA
Description: MOUSE ANTI HUMAN CD73
Specificity: CD73
Other names: ECTO-5'-NUCLEOTIDASE
Applications: E, WB
Format: Purified
Product Type: Monoclonal Antibody
Clone: 2B6
Isotype: IgG2b
Quantity: 0.1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting  1/1000 - 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Recombinant human CD73 (amino acids 27-252) purified from E. coli.
External Database Links
Click for detail UniProt:
P215895NTD_HUMANRelated reagents
Click for detail Entrez Gene:
4907NT5ERelated reagents
Click for detail GO:
GO:0005624membrane fractionRelated reagents
GO:0006259DNA metabolic processRelated reagents
GO:00082535'-nucleotidase activityRelated reagents
Synonyms
NT5, NTE
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse FO myeloma cell line.
Specificity
MCA4622GA specifically recognises human CD73, otherwise known as ecto-5’-nucleotidase, a GPI-anchored enzyme and member of the 5’-nucleotidase family, which catalyzes the dephosphorylation of 5’-mononucleotides into their corresponding nucleosides, and can also mediate co-stimulatory signals in T cell activation.

CD73 is expressed by subpopulations of T and B lymphocytes, and increases during lymphocyte development, acting as a lymphocyte maturation marker. A decrease in CD73 activity has been associated with several immunodeficiency disorders including AIDS and Wiskott-Aldrich syndrome, and CD73 is expressed on a variety of lymphomas and leukaemias, including CLL and ALL.
Western Blotting
MCA4622GA detects a band of approximately 63 kDa in human mesenchymal stem cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

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For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M138984:090825'


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Printed on 03 Sep 2010