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Datasheet: MCA4637T
Description: MOUSE ANTI HUMAN CD258
Specificity: CD258
Other names: TNFSF14
Applications: E, F
Format: Purified
Product Type: Monoclonal Antibody
Clone: T5-39
Isotype: IgG2b
Quantity: 25 µg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/10 - 1/20
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline pH7.2
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Immunogen
Recombinant human CD258
External Database Links
Click for detail UniProt:
O43557TNF14_HUMANRelated reagents
Click for detail Entrez Gene:
8740TNFSF14Related reagents
Click for detail GO:
GO:0005102receptor bindingRelated reagents
GO:0006917induction of apoptosisRelated reagents
GO:0008588release of cytoplasmic sequestered NF-kappaBRelated reagents
GO:0042098T cell proliferationRelated reagents
GO:0043027caspase inhibitor activityRelated reagents
GO:0043029T cell homeostasisRelated reagents
Synonyms
HVEML, LIGHT
Specificity
MCA4637T recognises human CD258, otherwise known as LIGHT (ligand for herpesvirus entry mediator), a 30kDa type 2 transmembrane member of the tumour necrosis factor ligand superfamily. It is a ligand for tumour necrosis factor receptor-like 2 (also known as herpesvirus entry mediator or HVEM). It also activates NFKB, stimulates the proliferation of T-cells and acts as a receptor for Herpes simplex virus. LIGTH inhibits the growth of some tumour cells and plays a role in intestinal inflammation. It is predominantly expressed in the spleen, but is also found in the brain and is weakly expressed in the heart, lung, liver, appendix and kidney.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Method sheets are available on request
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

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Goat Anti Mouse IgG (H/L) (STAR117...) Alk. Phos., DyLight®488, DyLight®549, DyLight®649, FITC, HRP
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Goat Anti Mouse IgG IgA IgM (STAR87...) Alk. Phos., HRP
Rabbit Anti Mouse IgG (STAR13...) HRP
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Goat Anti Mouse IgG (Fc) (STAR120...) FITC, HRP

Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M139030:090825'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 31 Jul 2010