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Datasheet: PHP200
Description: RECOMBINANT HUMAN RESISTIN
Name: RESISTIN
Other names: ADIPOSE TISSUE-SPECIFIC SECRETORY FACTOR
Applications: E, FN, WB
Format: Rec. Protein
Product Type: Recombinant Protein
Quantity: 25 µg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
ELISA  0.2 - 0.4ng/well
Western Blotting  1.5 - 3.0ng/lane
Functional Assays   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 250ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AbD Serotec recommend that the vial is gently mixed after reconstitution.
Preparation
Purified recombinant resistin expressed in E.coli.
Buffer Solution
10mM Sodium Citrate
Preservative Stabilisers
None present
Endotoxin Level
<1EU/ug
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution
External Database Links
Click for detail UniProt:
Q9HD89RETN_HUMANRelated reagents
Click for detail Entrez Gene:
56729RETNRelated reagents
Click for detail GO:
GO:0005179hormone activityRelated reagents
GO:0005576extracellular regionRelated reagents
Synonyms
FIZZ3, HXCP1, RSTN
Specificity
Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity.

Studies have shown that resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).
Molecular Weight
19.5kD
Activity
Determined by its ability to stimulate lipolysis in cultured human adipocytes.
Purity
>98% by SDS PAGE and HPLC analysis.
ELISA
PHP200 may used as a standard for ELISA applications involving AHP1618 or AHP1618B.
Western Blotting
PHP200 may be used as a positive control for western blotting application involving AHP1618 or AHP1618B.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.
Health And Safety Information
(A full Health and Safety assessment is available upon request)

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M126200:090602'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 29 Jul 2010