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Datasheet: AHP1010
Description: RABBIT ANTI HUMAN CD193
Specificity: CD193
Other names: CCR3
Applications: P, WB
Format: Purified
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin  10ug/ml
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting  1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Species Cross Reactivity
Reacts with: Rat, Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD193 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Immunogen
Synthetic peptide corresponding to a 14 amino acid sequence located near human CD193 carboxy-terminus.
External Database Links
Click for detail UniProt:
P51677CCR3_HUMANRelated reagents
Click for detail Entrez Gene:
1232CCR3Related reagents
Click for detail GO:
GO:0004950chemokine receptor activityRelated reagents
GO:0005887integral to plasma membraneRelated reagents
GO:0006935chemotaxisRelated reagents
GO:0006954inflammatory responseRelated reagents
GO:0006968cellular defense responseRelated reagents
GO:0007155cell adhesionRelated reagents
GO:0007188G-protein signaling, coupled to cAMP nucleotide second messengerRelated reagents
GO:0007204elevation of cytosolic calcium ion concentrationRelated reagents
Synonyms
CMKBR3
Specificity
AHP1010 recognises human CD193, otherwise known as CC chemokine receptor 3 (CCR3), a seven-helix transmembrane G-protein coupled receptor which, along with CCR5 and CXCR4, acts as a principal receptor for the fusion and entry of HIV into target cells. CD193 is expressed by eosinophils, basophils, mast cells, microglia, Th2 helper cells and some epithelial cells.

CD193 acts as a receptor for several CC chemokines including monocyte chemoattractant proteins (MCP) -1, -2, -3 and -4, MIP-1a, MIP-1ß, and RANTES. The eotaxin subgroup of CC chemokines act as specific agonists for CD193, and this interaction has become a focus area for studies into the infection of microglia by HIV (2) and also allergy research (3).
Histology Positive Control Tissue
Human spleen.
Western Blotting
AHP1010 detects a band of approximately 55kDa in human spleen cell lysates (predicted Mwt. 41kDa)
References
1. Combadiere, C. et al. (1995) Cloning and functional expression of a human eosinophil CC chemokine receptor*. J. Biol. Chem. 270: 16491-16494.
2. He, J. et al. (1997) CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 385: 645-649.
3. Holse, M. et al. (2006) CCR3, CCR5, CCR8 and CXCR3 expression in memory T helper cells from allergic rhinitis patients, asymptomatically sensitized and healthy individuals. Clin. Mol. Allergy. 4: 6.
4. Wainwright, D.A. et al. (2009) Exacerbation of facial motoneuron loss after facial nerve axotomy in CCR3-deficient mice. ASN Neuro. 1:e00024.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

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Recommended Positive Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M135381:090825'


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Printed on 31 Jul 2010