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Datasheet: AHP1043
Description: RABBIT ANTI HUMAN MIP-1 BETA
Specificity: MIP-1 BETA
Other names: CCL4
Applications: E, FN, WB
Format: Purified
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA  0.5ug/ml
Immunoprecipitation   
Western Blotting  0.1 - 0.2ug/ml
Functional Assays  1.75 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human MIP-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
None present
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Immunogen
Recombinant human MIP-1 beta.
External Database Links
Click for detail UniProt:
P13236CCL4_HUMANRelated reagents
Click for detail Entrez Gene:
6351CCL4Related reagents
Click for detail GO:
GO:0004716receptor signaling protein tyrosine kinase activityRelated reagents
GO:0005615extracellular spaceRelated reagents
GO:0006928cell motilityRelated reagents
GO:0006954inflammatory responseRelated reagents
GO:0006955immune responseRelated reagents
GO:0007155cell adhesionRelated reagents
GO:0007163establishment and/or maintenance of cell polarityRelated reagents
GO:0007165signal transductionRelated reagents
GO:0007267cell-cell signalingRelated reagents
GO:0008009chemokine activityRelated reagents
GO:0009615response to virusRelated reagents
GO:0019079viral genome replicationRelated reagents
Synonyms
CCL4L, LAG1, MIP1B, SCYA4, SCYA4L
Specificity
AHP1043 is specific for human MIP-1 beta, otherwise known as CCL4, a 69 amino acid member of the CC chemokine family, shown to signal through the chemokine receptors CCR1, CCR2 and CCR5.

MIP-1 beta is involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as monocytes, B cells, NK cells, neutrophils, dendritic cells and CD4+ lymphocytes.

The secretion of MIP-1 beta by CD8+ lymphocytes has been shown to act as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry.
References
1. Menten, P. et al. (2002) Macrophage inflammatory protein-1. Cyt. Growth Factor 13: 455-481.
2. Cocchi, F. et al. (1996) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells. Science 274: 1393-1395.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Health And Safety Information
(A full Health and Safety assessment is available upon request)

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Other formats and applications

This reagent is also available in a Biotin format

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M145402:091023'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 03 Sep 2010