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Datasheet: AHP395
Description: SHEEP ANTI HUMAN NF KAPPA B INHIBITOR ALPHA
Specificity: NF KAPPA B INHIBITOR ALPHA
Other names: IkB ALPHA
Applications: E, GS, IP, WB
Format: Purified
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA  1/2000
Immunoprecipitation   
Western Blotting  1/200 - 1/1000
Gel Super Shift Assays   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human NK kappa B inhibitor alpha was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Human NK kappa B inhibitor alpha expressed in E. coli
External Database Links
Click for detail UniProt:
P25963IKBA_HUMANRelated reagents
Click for detail Entrez Gene:
4792NFKBIARelated reagents
Click for detail GO:
GO:0005634nucleusRelated reagents
GO:0005737cytoplasmRelated reagents
GO:0006915apoptosisRelated reagents
GO:0007253cytoplasmic sequestering of NF-kappaBRelated reagents
GO:0008139nuclear localization sequence bindingRelated reagents
GO:0031625ubiquitin protein ligase bindingRelated reagents
GO:0043392negative regulation of DNA bindingRelated reagents
GO:0051059NF-kappaB bindingRelated reagents
Synonyms
IKBA, MAD3, NFKBI
Specificity
AHP395 is specific for NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB, and then ubiquinated prior to degradation.
Western Blotting
AHP395 detects a band of approximately 39 kDa in THPI cell lysates.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Secondary Antibodies

Rabbit Anti Sheep IgG (H/L) (5184-2304...) Biotin
Donkey Anti Sheep IgG (STAR88...) DyLight®488, DyLight®549, DyLight®649, DyLight®800, FITC, HRP

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M143052:091014'


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Printed on 03 Sep 2010