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Datasheet: AHP866
Description: RABBIT ANTI TIRAP
Specificity: TIRAP
Other names: MAL
Applications: P*, WB
Format: Purified
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin(1)   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting  0.5ug/ml - 2.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1)Special conditions apply. - See [*].
Target Species
Mouse
Species Cross Reactivity
Reacts with: Human, Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to mouse TIRAP were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Synthetic peptide corresponding to a region within the C-Terminus of mouse TIRAP.
External Database Links
Click for detail UniProt:
Q99JY1TIRAP_MOUSERelated reagents
Click for detail Entrez Gene:
117149TirapRelated reagents
Click for detail GO:
GO:0007166cell surface receptor linked signal transductionRelated reagents
GO:0007249I-kappaB kinase/NF-kappaB cascadeRelated reagents
GO:0030099myeloid cell differentiationRelated reagents
Synonyms
MAL
Specificity
AHP866 is specific for mouse TIRAP (toll-interleukin 1 receptor domain containing adaptor protein), also known as Mal.

TIRAP is one of a family of TIR domain-containing proteins which serve as adaptor proteins for the Toll-like receptors during the innate immune response. TIRAP is reported to participate in the MyD88-dependent signaling pathways shared by TLR2 and TLR4.
Immunohistology
* This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M134018:090817'


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Printed on 09 Sep 2010