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Datasheet: MCA1078
Description: MOUSE ANTI HORSE CD4
Specificity: CD4
Applications: F
Format: S/N
Product Type: Monoclonal Antibody
Clone: CVS4
Isotype: IgG1
Quantity: 2 ml
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  Neat
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Horse
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Equine Thymocytes
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Specificity
MCA1078 reacts with T-helper cells.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Lunn, D.P.et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunol. 74: 251-257.
2. Kydd, J.H. and Antczak, D.F., (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Equine Immunol. 4-5.
3. Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses. Invest Ophthalmol Vis Sci. 45: 2286-92
4. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Secondary Antibodies

Rabbit Anti Mouse IgG (STAR12...) RPE, RPE
Goat Anti Mouse IgG (H/L) (STAR117...) FITC
Rabbit Anti Mouse IgG (STAR9...) FITC

Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M141627:091014'


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Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
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Printed on 29 Jul 2010