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Datasheet: MCA1187
Description: MOUSE ANTI HUMAN CD49e
Specificity: CD49e
Other names: INTEGRIN ALPHA 5 CHAIN, VLA-5
Applications: C, F, IP
Format: Purified
Product Type: Monoclonal Antibody
Clone: JBS5
Isotype: IgG1
Quantity: 0.2 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  Neat - 1/10
Immunohistology - Frozen  0.5ug/ml - 1ug/ml
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation  1ug/ml - 5ug/ml
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Species Cross Reactivity
Reacts with: Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
External Database Links
Synonyms
FNRA
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity
MCA1187 reacts with the Alpha 5 integrin. Has been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines. It also inhibits the adherence of cells to fibronectin.

Removal of sodium azide is recommended prior to use in functional assays.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul of whole blood. Method sheets are available on request
Histology Positive Control Tissue
Human Endothelium
References
1. Shen, C. X. et al. (1991) Antibodies to different members of the beta 1 (CD29) Integrins induce homotypic and heterotypic cellular aggregation. Cell Immunol. 138: 216-228.
2. McCarty, O.J. et al. (2004) Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion. J Thromb Haemost. 2: 1823-1833.
3. Bax, D.V. et al. (2004) Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells. J Biol Chem. 279: 22377-22386.
4. Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo. Neoplasia. 11: 651-61.
5. Yao, H. et al. (2010) Increased potency of the PHSCN dendrimer as an inhibitorof human prostate cancer cell invasion, extravasation, and lung colony formation. Clin Exp Metastasis 27:173–84.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M141654:091014'


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Tel: +44 (0)1865 852700
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Printed on 03 Sep 2010