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Datasheet: MCA1218
Description: MOUSE ANTI PIG CD14
Specificity: CD14
Applications: C, F
Format: S/N
Product Type: Monoclonal Antibody
Clone: MIL-2
Isotype: IgG2b
Quantity: 2 ml
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/2 - 1/20
Immunohistology - Frozen  1/2 - 1/20
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Pig
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Porcine peripheral blood lymphocytes
Specificity
MCA1218 recognises porcine CD14. Recent studies suggest that two different epitopes of CD14 exist on pig cells and the distribution of these epitopes differ. One epitope is primarily expressed by monocytes and the second epitope is expressed by granulocytes, but is less accessible.

Clone MIL-2 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (2). Removal of sodium azide is recommended prior to use in functional assays. Serotec recommend the use of EQU003 for this purpose.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J. Imm. Methods. 170: 233-245.
2. Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers. Vet. Immunol. Immunopathol. 80: 93 - 109.
3. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs. FASEB J. 24: 712-22.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

Other formats and applications

This reagent is also available in a FITC format

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M145567:091028'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 02 Sep 2010