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Datasheet: MCA1477
Description: RAT ANTI HUMAN CD3
Specificity: CD3
Applications: C, F*, P*, WB
Format: Purified
Product Type: Monoclonal Antibody
Clone: CD3-12
Isotype: IgG1
Quantity: 0.2 mg
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Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD3 (MCA1477) following permeabilisation with Leucoperm (BUF09)
Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD3 (MCA1477) following permeabilisation with Leucoperm (BUF09)
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry(1)  1/50 - 1/100
Immunohistology - Frozen  1/1000
Immunohistology - Paraffin(2)  1/1000
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1) Special conditions apply - See [*].
(2) Special conditions apply - See [**].
Target Species
Human
Species Cross Reactivity
Reacts with: Bovine, Dog, Horse, Rhesus Monkey, Pig, Chicken, Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys)
External Database Links
Click for detail UniProt:
P07766CD3E_HUMANRelated reagents
Click for detail Entrez Gene:
916CD3ERelated reagents
Click for detail GO:
GO:0004888transmembrane receptor activityRelated reagents
GO:0005057receptor signaling protein activityRelated reagents
GO:0005887integral to plasma membraneRelated reagents
GO:0007172signal complex assemblyRelated reagents
GO:0007186G-protein coupled receptor protein signaling pathwayRelated reagents
GO:0009897external side of plasma membraneRelated reagents
GO:0017124SH3 domain bindingRelated reagents
GO:0019901protein kinase bindingRelated reagents
GO:0030159receptor signaling complex scaffold activityRelated reagents
GO:0042101T cell receptor complexRelated reagents
GO:0042102positive regulation of T cell proliferationRelated reagents
GO:0042608T cell receptor bindingRelated reagents
GO:0042981regulation of apoptosisRelated reagents
GO:0046982protein heterodimerization activityRelated reagents
Synonyms
T3E
Specificity
MCA1477 recognises a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.
Flow Cytometry
* Membrane permeabilisation is required for this application. AbD Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Immunohistology
** This product requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 1mM EDTA pH8.0 is recommended for this purpose.
Histology Positive Control Tissue
Tonsil
Recommended Reading
1. Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J. Immunol. 150: 5429-5435.
2. Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient- derived CD8 T cells. J. Virol. 72: 7733 - 7744.
3. Burudi, E. M. et al. (2002) Regulation of indoleamine 2, 3-dioxygenase expression in Simian Immunodeficiency Virus-infected monkey brains. J. Virol. 76: 12233 - 12241.
4. Shulga Morskaya, S. et al. (2004) B Cell-Activating Factor Belonging to the TNF Family Acts through Separate Receptors to Support B Cell Survival and T Cell-Independent Antibody Formation. J. Immunol. 173: 2331-2341.
5. Kapturczak, M. H. et al. (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse. Am. J. Pathol. 165: 1045-1053.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Image

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD3 (MCA1477) following permeabilisation with Leucoperm (BUF09)

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD3 (MCA1477). Permeabilised with Leucoperm

Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD3:RPE (MCA1477PE). Permeabilised with Leucoperm

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Recommended Negative Controls

Other formats and applications

This reagent is also available in a APC, Biotin, FITC, RPE format

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M141726:091014'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 09 Feb 2010