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Datasheet: MCA1482

Description: MOUSE ANTI HUMAN CD324
Specificity: CD324
Other names: E-CADHERIN
Applications: C, WB
Format: Purified
Product Type: Monoclonal Antibody
Clone: 6F9
Isotype: IgG1
Quantity: 1 ml
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen  1/10 - 1/40
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Ig fraction - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml
Immunogen
Human E-cadherin
External Database Links
Click for detail UniProt:
Click for detail Entrez Gene:
Click for detail GO:
GO:0005515protein bindingRelated reagents
GO:0007156homophilic cell adhesionRelated reagents
GO:0016021integral to membraneRelated reagents
Synonyms
CDHE, UVO
Specificity
MCA1482 recognises human E-cadherin, also known as CD324, an epithelial cell adhesion molecule located on the lateral border of epithelial cells.
References
1. Frixen, U.H. et al. (1991) E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells. J Cell Biol. 113: 173-85.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M129590:090728'