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Datasheet: MCA1544PE
Description: MOUSE ANTI HUMAN CD114:RPE
Specificity: CD114
Other names: G-CSF RECEPTOR
Applications: F
Format: RPE
Product Type: Monoclonal Antibody
Clone: LMM775
Isotype: IgG1
Quantity: 100 TESTS
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
External Database Links
Click for detail UniProt:
Q99062CSF3R_HUMANRelated reagents
Click for detail Entrez Gene:
1441CSF3RRelated reagents
Click for detail GO:
GO:0004872receptor activityRelated reagents
GO:0005887integral to plasma membraneRelated reagents
GO:0006952defense responseRelated reagents
GO:0007165signal transductionRelated reagents
Synonyms
GCSFR
Specificity
MCA1544PE recognises human CD114 (G-CSF receptor), expressed by peripheral blood granulocytes and some myeloid cell lines.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Method sheets are available on request.
References
1. Nicholson, S.E. et al. (1994) Tyrosine kinase JAK1 is associated with the granulocyte-colony-stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation. Proc. Natl. Acad. Sci. USA. 91: 2985-2988.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide : a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M129597:090728'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 31 Jul 2010