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Datasheet: MCA1584
Description: MOUSE ANTI HUMAN CD158b
Specificity: CD158b
Other names: KIR2DL3
Applications: F, IP
Format: Purified
Product Type: Monoclonal Antibody
Clone: GL183
Isotype: IgG1
Quantity: 0.2 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/10 - 1/50
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
NK cell clone E57
External Database Links
Click for detail UniProt:
P43628KI2L3_HUMANRelated reagents
Click for detail Entrez Gene:
3804KIR2DL3Related reagents
Click for detail GO:
GO:0003823antigen bindingRelated reagents
GO:0004872receptor activityRelated reagents
GO:0005887integral to plasma membraneRelated reagents
GO:0006955immune responseRelated reagents
Synonyms
CD158B2, KIRCL23, NKAT2
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3UI myeloma cell line.
Specificity
MCA1584 recognises the human CD158b cell surface antigen, a 58kD glycoprotein expressed by a subset of NK cells (also known as p58.2 antigen).

This antibody also recognises a 50kD molecule, which is highly homologous to p58.2 in the extracellular domain, but has a shorter cytoplasmic tail.

Both molecules are members of the newly described natural killer cell receptor family.

CD158b functions as a receptor specific for HLA Class I molecules, including Cw3 and related HLA-C alleles. This antibody can restore the lysis by human NK clones of otherwise lysis protected targets expressing Cw3.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Moretta, A. et al. (1990) A novel surface antigen expressed by a subset of human CD3-CD16+ natural killer cells. J. Exp. Med. 171: 695 - 714.
2. Moretta, A. et al. (1993) p58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells. Anti-p58 antibodies reconstitute lysis of MHC class-I protected cells in NK clones displaying different specificities. J. Exp. Med. 178: 597-604.
3. Ciccone, E. et al. (1992) Evidence of a natural killer (NK) cell repertoire for (allo) antigen recognition: definition of five distinct NK-determined allospecificities in humans. J. Exp. Med. 175: 709-718.
4. Moretta, A. et al. (1997) Major histocompatibility complex class I - specific receptors on human natural killer and T lymphocytes. Immunol Rev. 155: 105-117.
5. Moretta, A. et al. (1995) Existence of both inhibitory (p58) and activatory (p50) receptors for HLA-C molecules in human natural killer cells. J. Exp. Med. 182: 875-884.
6. Biassoni, R. et al. (1996) The human leukocyte antigen (HLA)-C-specific "activatory" or "inhibitory" natural killer cell receptors display highly homologous extracellular domains but differ in their transmembrane and intracytoplasmic portions. J. Exp. Med. 183: 645-650.
7. Long, E.O. et al. (1996) Inhibitory MHC Class I receptors on NK and T cells: a standard nomenclature. Immunol. Today 17: 100.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M158689:100721'


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Printed on 03 Sep 2010