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Datasheet: MCA1725F
Description: MOUSE ANTI HUMAN CD16b:FITC
Specificity: CD16b
Other names: FcRIII B
Applications: F
Format: FITC
Product Type: Monoclonal Antibody
Clone: 1D3
Isotype: IgM
Quantity: 100 TESTS
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  Neat
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Human granulocytes
External Database Links
Click for detail UniProt:
O75015FCG3B_HUMANRelated reagents
Click for detail Entrez Gene:
2215FCGR3BRelated reagents
Click for detail GO:
GO:0006955immune responseRelated reagents
Synonyms
CD16B, FCG3, FCGR3, IGFR3
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity
MCA1725F recognises the human CD16b cell surface antigen.

CD16b is the GPI - linked FcRIIIB molecule, which is a low affinity receptor for human IgG.

CD16b is expressed only by neutrophils, whilst CD16a is expressed by neutrophils and natural killer cells.
Flow Cytometry
Use 20ul of the suggested working dilution to label 5x105 cells in 100ul. Method sheets are available on request.
References
1. Ravetch, J.V. and Kinet, J.D. (1991) Fc receptors. Ann. Rev. Immunol. 9: 457-492.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M158096:100702'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 31 Jul 2010