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Datasheet: MCA1820
Description: MOUSE ANTI BOVINE INTERLEUKIN-4
Specificity: IL-4
Applications: E, F*
Format: Purified
Product Type: Monoclonal Antibody
Clone: CC303
Isotype: IgG2a
Quantity: 0.5 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry(1)  1/50 - 1/100
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.

(1) Special conditions apply. - See [*].
Target Species
Bovine
Species Cross Reactivity
Reacts with: Dog, Pig, Sheep, Mustelid, Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
External Database Links
Click for detail UniProt:
P30367IL4_BOVINRelated reagents
Click for detail Entrez Gene:
280824IL4Related reagents
Click for detail GO:
GO:0005615extracellular spaceRelated reagents
GO:0030890positive regulation of B cell proliferationRelated reagents
GO:0042102positive regulation of T cell proliferationRelated reagents
GO:0042325regulation of phosphorylationRelated reagents
GO:0043066negative regulation of apoptosisRelated reagents
GO:0045348positive regulation of MHC class II biosynthetic processRelated reagents
GO:0045671negative regulation of osteoclast differentiationRelated reagents
GO:0045944positive regulation of transcription from RNA polymerase II promoterRelated reagents
GO:0048295positive regulation of isotype switching to IgE isotypesRelated reagents
GO:0048304positive regulation of isotype switching to IgG isotypesRelated reagents
GO:0050776regulation of immune responseRelated reagents
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity
MCA1820 recognises bovine interleukin 4
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.

*Membrane permeabilisation is required for this application. AbD Serotec recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.

Please note, species cross reactivity can be unreliable in flow cytometry.
References
1. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross react with cytokines from different animal species. Vet. Immunol. Immunopathol. 88: 111 - 122.
2. Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus. Clin. Diagn. Lab. Immunol. 9: 1229 - 1234.
3. Nielsen, L. et al. (2009) Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host. J Gen Virol. 90: 2157-65.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Goat Anti Mouse IgG (STAR76...) RPE
HuCAL Anti Mouse IgG2a (HCA037...) FITC, HRP
Goat Anti Mouse IgG (STAR70...) FITC
Goat Anti Mouse IgG (H/L) (STAR117...) Alk. Phos., DyLight®488, DyLight®549, DyLight®649, FITC, HRP
Goat Anti Mouse IgG (STAR77...) HRP
Goat Anti Mouse IgG IgA IgM (STAR87...) Alk. Phos., HRP
Rabbit Anti Mouse IgG (STAR13...) HRP
Rabbit Anti Mouse IgG (STAR9...) FITC
Goat Anti Mouse IgG (Fc) (STAR120...) FITC, HRP

Recommended Negative Controls

Other formats and applications

This reagent is also available in a RPE format

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M136960:090825'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 29 Jul 2010