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Datasheet: MCA1853
Description: MOUSE ANTI HUMAN CD163
Specificity: CD163
Applications: C, F, P, WB
Format: Purified
Product Type: Monoclonal Antibody
Clone: EDHu-1
Isotype: IgG1
Quantity: 0.2 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Species Cross Reactivity
Reacts with: Rhesus Monkey, Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline pH7.4
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Immunogen
Leucocytes harvested from pleural cavity of patients with idiopathic spontaneous pneumothorax
External Database Links
Click for detail UniProt:
Q86VB7C163A_HUMANRelated reagents
Click for detail Entrez Gene:
9332CD163Related reagents
Click for detail GO:
GO:0005044scavenger receptor activityRelated reagents
GO:0005515protein bindingRelated reagents
GO:0005576extracellular regionRelated reagents
GO:0005887integral to plasma membraneRelated reagents
Synonyms
M130
Specificity
MCA1853 recognises the human CD163 cell surface antigen, a 130-140kD glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone.

Clone EDHu-1 is reported to inhibit the binding of haptoglobin.haemoglobin to CD163 (2). Removal of sodium azide is recommended prior to use in functional studies, Serotec recommend the use of EQU003 for this purpose.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor. Nature 409: 198-201.
2. Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region. J. Biol. Chem. 279: 51561-51567.
3. Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am. J. Pathol. 168: 822-834.
4. Moreno, J.A. et al. (2010) Peripheral Artery Disease Is Associated With a High CD163/TWEAK Plasma Ratio. Arterioscler Thromb Vasc Biol. 2010 Mar 18. [Epub ahead of print]
5. Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep. Vet Pathol. 47: 518-28.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

Other formats and applications

This reagent is also available in a FITC format

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M153138:100505'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 06 Sep 2010