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Datasheet: MCA1981

Description: MOUSE ANTI HUMAN CD240DCE
Specificity: CD240
Other names: RHESUS 30
Applications: F, IP
Format: Purified
Product Type: Monoclonal Antibody
Clone: BRIC69
Isotype: IgG1
Quantity: 0.2 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Tris buffered glycine, pH7.7
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Immunogen
Human erythrocytes
External Database Links
Click for detail UniProt:
Click for detail Entrez Gene:
Click for detail GO:
GO:0005887integral to plasma membraneRelated reagents
Synonyms
RHC, RHE
Specificity
MCA1981 recognises human erythrocytes of normal Rh types but does not recognise Rhnull erythrocytes. MCA1981 reacts with 31kD and 35-52kD proteins which were designated CD240DCE at the 7th leucocyte typing workshop.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Mouro-Chanteloup I. et al. (2002) Cell-surface expression of RhD blood group polypeptide is transcriptionally regulated by the RhAG glycoprotein. Blood 100:1038-1047
2. Mouro-Chanteloup. I. et al. (2003) Evidence that the red cell skeleton protein 4.2 interacts with the Rh membrane complex member CD47. Blood 101:338-344
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M134275:090817'