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Datasheet: MCA1982
Description: MOUSE ANTI HUMAN CD239
Specificity: CD239
Other names: B-CAM
Applications: F, WB
Format: Purified
Product Type: Monoclonal Antibody
Clone: BRIC221
Isotype: IgG2b
Quantity: 0.2 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
35 mM TRIS, 100 mM glycine and 150 mM NaCl pH 7.6
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Human erythrocytes
External Database Links
Click for detail UniProt:
P50895LU_HUMANRelated reagents
Click for detail Entrez Gene:
4059BCAMRelated reagents
Click for detail GO:
GO:0004888transmembrane receptor activityRelated reagents
GO:0005887integral to plasma membraneRelated reagents
GO:0007155cell adhesionRelated reagents
GO:0007165signal transductionRelated reagents
GO:0009986cell surfaceRelated reagents
Synonyms
LU, MSK19
Specificity
MCA1982 recognises a non-polymorphic determinant on both the 85 and 78kD Lutheran (Lu) glycoproteins. BRIC 221 specifically recognises an epitope in the fourth extracellular domain of Lu glycoprotein. Lutheran glycoprotein is a member of the immunoglobulin superfamily and has recently been designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Parsons, S.F. et al. (1997) Use of Domain-deletion mutants to locate Lutheran Blood Groups Antigens to each of the five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein; Elucidation and the Aua/Aub Polymorphisms. Blood 89: 4219-4225.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M134277:090817'


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Printed on 31 Jul 2010