Jump to content
Your first choice for antibodies!
AbD Serotec Title Image

Datasheet: MCA333S
Description: CHIMAERIC HUMAN IgE ANTI NP
Specificity: CHIMAERIC HUMAN IgE ANTI NP
Applications: E
Format: S/N
Product Type: Monoclonal Antibody
Clone: JW8/1
Isotype: IgE
Quantity: 2 ml
Price:
(Select Country)
Add to basket
Request Bulk Pricing
Inquiries/Feedback
Download as PDF

Related products

Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA  1/20 - 1/100
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgE concentration 0.02 - 0.05 mg/ml
Immunogen
Hapten, 4-hydroxy-3-nitrophenylacetyl (NP)
Fusion Partners
Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
Specificity
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimaeric human IgE antibody specific for NP has been produced.
References
1. Neuberger, M. S. et al. (1984) Recombinant antibodies possessing novel effector functions. Nature 312: 604-608.
2. Neuberger, M. S. et al. (1985) Making novel antibodies by expressing transfected immunoglobulin genes. TIBS 347-349.
3. Neuberger, M. S. et al. (1985) A hapten-specific chimaeric IgE antibody with Human physiological effector function. Naure 314: 268-270.
4. Neuberger, M. S. et al. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Phil. Trans. R. Soc. Lond. A317: 425-432.
5. Sayers, I. et al. (2004) The importance of Lys-352 of human immunoglobulin E in FcepsilonRII/CD23 recognition. J Biol Chem. 279: 35320-5.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M142101:091014'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 06 Sep 2010