Jump to content
Your first choice for antibodies!
AbD Serotec Title Image

Datasheet: MCA334B

Description: CHIMAERIC HUMAN IgG2 ANTI NP
Specificity: IgG2 ANTI NP (CHIMAERIC)
Applications: E
Format: Purified
Product Type: Monoclonal Antibody
Clone: JW183
Isotype: IgG2
Quantity: 0.5 mg
Price:
(Select Country)

Related products

Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
ELISA   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
1 mg/ml
Specificity
The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimaeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. The J558L cell-line itself secretes a lambda light chain but no heavy chain. Thus a chimaeric human IgG2 antibody specific for NP has been produced.
References
1. Neuberger, M.S. et al. (1984) Recombinant antibodies possessing novel effector functions. Nature 312: 604-608.
2. Neuberger, M.S. et al. (1985) A hapten-specific chimaeric IgE antibody with human physiological effector function. Nature 314: 268-270.
3. Neuberger, M.S. et al. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Trans. R. Soc. Lond. A317: 425-432.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M142102:091014'