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Datasheet: MCA694
Description: MOUSE ANTI HUMAN CD1c
Specificity: CD1c
Applications: C, F, IP
Format: Purified
Product Type: Monoclonal Antibody
Clone: L161
Isotype: IgG1
Quantity: 0.2 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA   
Immunoprecipitation   
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Thymus cells
External Database Links
Click for detail UniProt:
P29017CD1C_HUMANRelated reagents
Click for detail Entrez Gene:
911CD1CRelated reagents
Click for detail GO:
GO:0005887integral to plasma membraneRelated reagents
Fusion Partners
Spleen cells from immunised Biozzi mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity
MCA694 recognises a polypeptide (CD1c) antigen of 43kD which is associated with the 12kD beta 2 microglobulin present on thymocytes, a subpopulation of B-lymphocytes early during ontogeny, Langerhans cells and dendritic cells. It may be useful for studies of calcium flux induced by CD1c.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Method sheets are available on request.
References
1. Amiot, M. et al. (1986) Heterogeneity of the first cluster of differentiation: characterization and epitopic mapping of three CD1 molecules on normal human thymus cells. J. Immunol. 136: 1752-1758.
2. Amiot, M. et al. (1987) Analysis of CD1 molecules on thymus cells and leukemic T lymphoblasts identifies discrete phenotypes and reveals that CD1 intermolecular complexes are observed only on normal cells. Blood. 70 (3): 676-685.
3. Amiot, M. et al. (1988) Intermolecular complexes between three human CD1 molecules on normal thymus cells. Immunogenetics 27: 187-195.
4. Baumsell, L. et al. (1989) Cluster report: CD1 Leucocyte Typing IV. Oxford University Press, Oxford.251 - 254.
5. Theodorou, I.D.et al. (1990) CD1 stimulation of human T-cell lines induces a rapid increase in the intracellular free Ca2+ concentration and the production of IL-2. J. Immunol. 144 (7): 2518 - 2523.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

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Recommended Negative Controls

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M126111:090522'


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Printed on 31 Jul 2010