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Datasheet: PEP005
Description: RECOMBINANT HORSE INTERLEUKIN-8
Name: IL-8
Other names: CXCL8
Applications: FN
Format: Rec. Protein
Product Type: Recombinant Protein
Quantity: 20 µg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
ELISA   
Functional Assays   
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
Target Species
Horse
Product Form
Purified recombinant protein - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water.
Note
Further dilutions should be made in a buffer containing carrier protein.

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Serotec recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in pichia pastoris
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
External Database Links
Click for detail UniProt:
O62812IL8_HORSERelated reagents
Click for detail Entrez Gene:
100037400IL8Related reagents
Specificity
Equine interleukin 8 (IL-8) is principally produced by macrophages and is involved in neutrophil chemotaxis.

PEP005 has an ED50 in the range 20-25 ng/ml determined by studies of the induction of equine IL-8 on neutrophil migration in vitro.
Protein Molecular Weight
8.5kD
Purity
>95% by SDS PAGE analysis
References
1. Van de Walle, G.R.et al. (2009) Analysis of the herpesvirus chemokine-binding glycoprotein G residues essential for chemokine binding and biological activity. J Biol Chem. 284: 5968-76.
2. Van de Walle, G.R.et al. (2007) Herpesvirus chemokine-binding glycoprotein G (gG) efficiently inhibits neutrophil chemotaxis in vitro and in vivo. PubMed PMID: J Immunol. 179: 4161-9.
Storage
Prior to reconstitution store at +4oC.
Following reconstitution store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Health And Safety Information
(A full Health and Safety assessment is available upon request)

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M139767:090825'


AbD Serotec offices contact details click here

Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 29 Jul 2010