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Datasheet: STAR105F
Description: GOAT F(ab')2 ANTI MOUSE IgG (H/L):FITC
Specificity: IgG (H/L)
Applications: F
Format: FITC
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.7 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/100 - 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Mouse
Species Cross Reactivity
Reacts with: Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to Mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline pH7.2
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
F(ab')2 concentration 0.7mg/ml
Immunogen
Purified mouse IgG
External Database Links
Click for detail UniProt:
P01869IGH1M_MOUSERelated reagents
P01843LAC1_MOUSERelated reagents
P01724LV1B_MOUSERelated reagents
P01867GCBM_MOUSERelated reagents
P01864GCAB_MOUSERelated reagents
P01868IGHG1_MOUSERelated reagents
P01865GCAM_MOUSERelated reagents
P01837KAC_MOUSERelated reagents
P01844LAC2_MOUSERelated reagents
P03987GC3M_MOUSERelated reagents
P01863GCAA_MOUSERelated reagents
P01845LAC3_MOUSERelated reagents
Click for detail Entrez Gene:
16017Ighg1Related reagents
433053LOC433053Related reagents
16016Igh-3Related reagents
16017Ighg1Related reagents
16142Igl-V1Related reagents
380793Igh-1aRelated reagents
16071Igk-CRelated reagents
110786Igl-C2Related reagents
110787Igl-C3Related reagents
380793Igh-1aRelated reagents
380793Igh-1aRelated reagents
380795AI324046Related reagents
Click for detail GO:
GO:0001788antibody-dependent cellular cytotoxicityRelated reagents
GO:0001798positive regulation of type IIa hypersensitivityRelated reagents
GO:0001812positive regulation of type I hypersensitivityRelated reagents
GO:0003823antigen bindingRelated reagents
GO:0005771multivesicular bodyRelated reagents
GO:0006910phagocytosis, recognitionRelated reagents
GO:0006911phagocytosis, engulfmentRelated reagents
GO:0006958complement activation, classical pathwayRelated reagents
GO:0008333endosome to lysosome transportRelated reagents
GO:0019731antibacterial humoral responseRelated reagents
GO:0019882antigen processing and presentationRelated reagents
GO:0030162regulation of proteolysisRelated reagents
GO:0042571immunoglobulin complex, circulatingRelated reagents
GO:0045022early endosome to late endosome transportRelated reagents
GO:0050766positive regulation of phagocytosisRelated reagents
GO:0050871positive regulation of B cell activationRelated reagents
Specificity
STAR105F has been shown to react with all mouse IgG subclasses in gel immunodiffusion.

It is adsorbed against human immunoglobulins. Cross-reactivity with rat IgG is expected.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Hsiao, Y. et al. (2002) Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible Venereal tumor cells. Vet. Immunol. Immunopathol. 87: 19-27.
2. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow. J Pharmacol Exp Ther 321: 656-662.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Other formats and applications

This reagent is also available in a HRP, Purified, RPE format and is useful for Immunohistology - Frozen, Immunohistology - Paraffin, Immunohistology - Resin, ELISA, Western Blotting

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M80761:090422'


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Email: tech.uk@abdserotec.com
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Printed on 06 Sep 2010