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Datasheet: STAR108A
Description: RABBIT F(ab')2 ANTI MOUSE IgG:Alk. Phos.
Specificity: IgG
Applications: C, E, P, WB
Format: Alk. Phos.
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 0.5 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry   
Immunohistology - Frozen   
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA  1/1000
Immunoprecipitation   
Western Blotting   
Radioimmunoassays   
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Target Species
Mouse
Species Cross Reactivity
Reacts with: Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
F(ab')2 fragment of rabbit IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove the remaining intact IgG or Fc fragments.
Buffer Solution
TRIS buffered saline pH8.0
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
25%Glycerol
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Immunogen
Mouse IgG
External Database Links
Click for detail UniProt:
P01869IGH1M_MOUSERelated reagents
P01865GCAM_MOUSERelated reagents
P03987GC3M_MOUSERelated reagents
P01867GCBM_MOUSERelated reagents
P01864GCAB_MOUSERelated reagents
P01868IGHG1_MOUSERelated reagents
P01863GCAA_MOUSERelated reagents
Click for detail Entrez Gene:
16017Ighg1Related reagents
380793Igh-1aRelated reagents
16016Igh-3Related reagents
16017Ighg1Related reagents
380793Igh-1aRelated reagents
380795AI324046Related reagents
380793Igh-1aRelated reagents
Click for detail GO:
GO:0001788antibody-dependent cellular cytotoxicityRelated reagents
GO:0001798positive regulation of type IIa hypersensitivityRelated reagents
GO:0001812positive regulation of type I hypersensitivityRelated reagents
GO:0003823antigen bindingRelated reagents
GO:0005771multivesicular bodyRelated reagents
GO:0006910phagocytosis, recognitionRelated reagents
GO:0006911phagocytosis, engulfmentRelated reagents
GO:0006958complement activation, classical pathwayRelated reagents
GO:0008333endosome to lysosome transportRelated reagents
GO:0019731antibacterial humoral responseRelated reagents
GO:0019882antigen processing and presentationRelated reagents
GO:0030162regulation of proteolysisRelated reagents
GO:0042571immunoglobulin complex, circulatingRelated reagents
GO:0045022early endosome to late endosome transportRelated reagents
GO:0050766positive regulation of phagocytosisRelated reagents
GO:0050871positive regulation of B cell activationRelated reagents
Specificity
STAR108A recognises mouse IgG.

Cross reactivity is observed with rat IgG. Cross-reactivity with human IgG has been minimised by solid phase absorption.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M145251:091014'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 06 Sep 2010