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Datasheet: STAR21B

Description: RABBIT F(ab')2 ANTI RAT IgG:HRP
Specificity: IgG
Applications: C, E, P, RE
Format: HRP
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 1 mg
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Immunohistology - Frozen  1/50
Immunohistology - Paraffin  1/50
Immunohistology - Resin  1/50
ELISA  1/500 - 1/1000
Western Blotting   
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Rat
Species Cross Reactivity
Reacts with: Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography of serum
Buffer Solution
Phosphate buffered saline pH7.2
Preservative Stabilisers
HRP stabiliser (BUF052A)
0.01% Thiomersal
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Purified rat immunoglobulin G
External Database Links
Specificity
Sera from rabbits hyperimmunised with highly purified rat IgG was affinity purified on a rat IgG column. Digestion to F(ab')2 with pepsin was then performed, the resulting fragments were conjugated to horseradish peroxidase using the periodate method.

Cross reactivity is observed with mouse IgG but may be blocked by the addition of 10% mouse serum.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains thiomersal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M145267:091014'