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Datasheet: STAR73
Description: GOAT F(ab')2 ANTI RAT IgG:RPE (MOUSE ADSORBED)
Specificity: IgG
Applications: F
Format: RPE
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 100 TESTS
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry  1/5 - 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Rat
Product Form
F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
F(ab')2 concentration 0.2 mg/ml
External Database Links
Specificity
STAR73 recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues.

We recommend diluting this product in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul. Method sheets are available on request.
References
1. Bellingan. G. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J. Exp. Med. 196:1515-1521.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M81506:090422'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 06 Sep 2010