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Datasheet: STAR88P
Description: DONKEY ANTI SHEEP/GOAT IgG:HRP
Specificity: IgG
Applications: C, E, P, WB
Format: HRP
Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Quantity: 1 ml
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Product Details

Applications
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Immunohistology - Frozen  1/50 - 1/100
Immunohistology - Paraffin   
Immunohistology - Resin   
ELISA  1/12800 - 1/25600
Western Blotting  1/20000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Sheep
Species Cross Reactivity
Reacts with: Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to sheep IgG were raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline pH7.2
Preservative Stabilisers
0.05%ProclinTM 300
0.002%Methylisothiazolone
0.002%Bromonitrodioxane
Approx. Protein Concentrations
1.0 mg/ml
Immunogen
Sheep IgG
Specificity
STAR88P recognises both sheep and goat IgG, which are immunologically very similar.

STAR88P is adsorbed against human, rabbit, mouse and rat serum proteins to minimise cross-reactivity.
References
1. Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice. J. Virol. 73 (6): 4823 - 4828.
2. Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP) - 1 alpha and MIP-1 beta. J. Immunol. 161: 5663 - 5672
3. Turner, J. et al. (2002) in vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice. J. Immunol. 169: 6343 - 6351.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Health And Safety Information
(A full Health and Safety assessment is available upon request) This product contains ProclinTM 300, Methylisothiazolone, Bromonitrodioxane: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Other formats and applications

This reagent is also available in a Alk. Phos., DyLight®405, DyLight®488, DyLight®549, DyLight®649, DyLight®800, FITC format and is useful for Flow Cytometry, Immunofluorescence

For research purposes only, unless otherwise specified in writing by AbD Serotec.

'M151004:100311'


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Address:
MorphoSys UK Ltd, Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxford, OX5 1GE, UK

Tel: +44 (0)1865 852700
Email: tech.uk@abdserotec.com
Web: www.abdserotec.com

Printed on 03 Sep 2010