DyLight® Dyes

Newborn Rat Primary cardiac Myocytes stained with rabbit anti MyBP-C detected with Sheep Anti Rabbit IgG:Dylight® 594 (STAR71D649) and mouse anti alpha actin, detected with Goat Anti Mouse IgG: Dylight® 488 (STAR74D488)

DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy.

  • More intense fluorescence and sensitivity than comparable spectral conjugates
  • Greater photostability than other fluorochromes
  • Good stability over pH 4 - 9
  • Ideal for immunofluorescence techniques
  • Compatible with all common fluorescence detection channels
  • Available for custom conjugation

DyLight® 405
DyLight® 488
DyLight® 800

DyLight dyes are excellent alternatives to Alexa Fluor®, CyDye® and LI-COR® fluorescent dyes. DyLight dyes provide intense fluorescence and sensitivity requiring less conjugated antibody to study your cells than FITC and RPE, saving you money.

They are primarily suitable for use in immunofluorescence techniques such as microscopy and flow cytometry, and may also be used in ELISA, Western blotting, high throughput screening and other array platforms.

Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.

We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice.

Dye Laser
excitation
line (nm)
Maximal absorbance (nm) Maximal emission (nm) Suggested laser Fluores- cence
color
DyLight® 405 400 405 420 Violet diode  
DyLight® 488 493 488 518 Argon-Ion  
DyLight® 800 770 800 794 Argon-Ion  

DyLight® 405

  • Exceptionally resistant to photo-bleaching and is an ideal blue fluorophore for demanding fluorescence conditions
  • Equivalent to Alexa Fluor® 405 and Cascade blue dyes

dy405

DyLight 405 Dye: Absorption (black), Emission (blue)


DyLight® 488

  • Outperforms FITC and Cy2 with fluorescent brightness, photostability and pH stability
  • Equivalent to Alexa Fluor® 488, fluorescein and FITC dyes

dy488

DyLight® 488 Dye: Absorption (black), Emission (green)


DyLight® 800

  • Outperforms IRDye® 800 with extremely low autofluorescence – ideal for in vivo imaging
  • Compatible for use in the LI-COR® Odyssey Infrared Imaging System

dy800

DyLight® 800 Dye: Absorption (black), Emission (brown, infrared)


We offer custom conjugations of these DyLight® dyes to any of our primary antibodies or to your antibody. Not all antibody suppliers can offer you this Custom Service, just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.

Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.
CyDye® is a trademark of GE Healthcare.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
LI-COR® and IRDye® 800 are trademarks of LI-COR Biosciences.

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