Newborn Rat Primary cardiac Myocytes stained with rabbit anti MyBP-C detected with Sheep Anti Rabbit IgG:Dylight® 594 (STAR71D649) and mouse anti alpha actin, detected with Goat Anti Mouse IgG: Dylight® 488 (STAR74D488)
DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy.
DyLight dyes are excellent alternatives to Alexa Fluor®, CyDye® and LI-COR® fluorescent dyes. DyLight dyes provide intense fluorescence and sensitivity requiring less conjugated antibody to study your cells than FITC and RPE, saving you money.
They are primarily suitable for use in immunofluorescence techniques such as microscopy and flow cytometry, and may also be used in ELISA, Western blotting, high throughput screening and other array platforms.
Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.
We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice.
|Maximal absorbance (nm)||Maximal emission (nm)||Suggested laser||
|DyLight® 405||400||405||420||Violet diode|
DyLight 405 Dye: Absorption (black), Emission (blue)
DyLight® 488 Dye: Absorption (black), Emission (green)
DyLight® 800 Dye: Absorption (black), Emission (brown, infrared)
We offer custom conjugations of these DyLight® dyes to any of our primary antibodies or to your antibody. Not all antibody suppliers can offer you this Custom Service, just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.
Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.
CyDye® is a trademark of GE Healthcare.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
LI-COR® and IRDye® 800 are trademarks of LI-COR Biosciences.