HuCAL® References

Back, J.W.  et al. (2012) Selecting Highly Structure-specific Antibodies using Structured Synthetic Mimics of the Cystine Knot Protein Sclerostin.
Protein Engineering, Design & Selection pp. 1-9, 2012 [Epub ahead of print]

Petrul, H.M. et al. (2012) Therapeutic Mechanism and Efficacy of the Antibody-Drug Conjugate BAY 79-4620 Targeting Human Carbonic Anhydrase 9.
Mol Cancer Ther. 2012 Feb;11(2):340-9 [Epub ahead of print]

Ostrowitzki, S. et al. (2012) Mechanism of Amyloid Removal in Patients With Alzheimer Disease Treated With Gantenerumab.
Arch Neurol. 2011 Oct 10 [Epub ahead of print]

Bohrmann, B. et al. (2012) Gantenerumab: A Novel Human Anti-Aβ Antibody Demonstrates Sustained Cerebral Amyloid-β Binding and Elicits Cell-Mediated Removal of Human Amyloid-β
J Alzheimers Dis. 2011 Sep 28 [Epub ahead of print]

Whelan, O.W. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 2011 Dec 22 e29194 [Epub ahead of print]

Giblin, P. et al. (2011) Fully Human Antibodies against the Protease-Activated Receptor-2 (PAR-2) with Anti-inflammatory Activity.
Hum Antibodies. 2011; 20(3-4):83-94

Prassler, J. et al. (2011) HuCAL PLATINUM, a Synthetic Fab Library Optimized for Sequence Diversity and Superior Performance in Mammalian Expression Systems.
J. Mol Biol. 413:261-78

Schwaeble, W.J. et al. (2011) Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury.
Proc Natl Acad Sci U S A. 108:7523-8

Kwong, L.S. et al. (2011) Characterisation of antibodies to bovine Toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2.
Vet Immunol Immunopathol. 139:313-8

Casrouge, A. et al. (2011) Evidence for an antagonist form of the chemokine CXCL10 in patients chronically infected with HCV.
J Clin Invest. 121:308-17

Rankl, C. et al. (2010) Detection of corneodesmosin on the surface of stratum corneum using atomic force microscopy.
Exp Dermatol. 19:1014-1019

Gustchina, E. et al. (2010) Structural Basis of HIV-1 Neutralization by Affinity Matured Fabs Directed against the Internal Trimeric Coiled-Coil of gp41.
PLoS Pathog. 6:e1001182

Ogawa, S. et al. (2010) Anti-PDGF-B monoclonal antibody reduces liver fibrosis development.
Hepatology Res. 40:1128–1141

Harth, S. et al. (2010) A Selection Fit Mechanism in BMP Receptor IA as a Possible Source for BMP Ligand-Receptor Promiscuity.
PLoS One. 28;5(9). pii: e13049

Larkin, E., Stiles, B., and Ulrich, R. (2010) Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B.
PLoS One. 5(10):e13253

Harth, S. et al. (2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
Acta Cryst. F66:964–968

Moore, C. (2010) Using recombinant monoclonal antibodies to develop in vitro diagnostics. IVD Technology.
IVD Technology 16:25-32

Shiryaev, S. A. et al. (2010) Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
Biochem J. 427:369-76?

Summer, A. et al. (2010) Short Communication: Characterization of a monoclonal antibody for K-Casein B of cow’s milk.
J Dairy Sci. 93:796-800

Okerlund, N. D. et al. (2010) Dact1 Is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain
J Neurosci. 30:4362-8?

Ohashi, M., Eagle, R. A., and Trowsdale, J. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a GPI-linked isoform.
J Biol Chem. Mar 19. Epub ahead of print? .

Vallon, M. et al. (2010) Tumor endothelial marker 5 expression in endothelial cells during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of cell proliferation.
Exp Cell Res. 316:412-421?

Härtle, S. et al (2009) Fast development of highly specific, fully human anti-idiotypic antibodies.
Abstracts of IBC’s 7th Annual International Conference on Antibody Therapeutics: 2009 December 8-10, San Diego, California.

Bottos, A. et al. (2009) The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions.
Proc Natl Acad Sci U S A. Nov 19. [Epub ahead of print]

Prassler, J. et al. (2009) In vitro affinity maturation of HuCAL antibodies: complementarity determining region exchange and RapMAT technology.
Future Medicine, Immunotherapy. 1:574-583?

Ylera, F. et al. (2009) HuCAL PLATINUM® - The new source for diagnostic and research antibodies,
Abstracts of the Society for Biomolecular Sciences 15th Annual Conference and Exhibition: 2009 April 26-??30. Lille, France

Gustchina, E. et al. (2009) Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
Virology. 39:112-119.

Knappik, A. et al. (2009) Development of recombinant human IgA for anticardiolipin antibodies assay standardization.
Ann N Y Acad Sci. 1173:190-198.

Knappik, A. and Brundiers, R. (2009) Recombinant antibody expression and purification. In: Walker, J.M. editor. The Protein Protocols Handbook. 3rd edition.
New York: Humana Press Inc.

Teplyakov, A. et al. (2009) Epitope mapping of anti-interleukin-13 neutralizing antibody CNTO607.
J Mol Biol. 389:115-23.

Jung, S.M., Tsuji, K., and Moroi, M. (2009) Glycoprotein (GP) VI dimer as a major collagen-binding site of native platelets: direct evidence obtained with dimeric GPVI-specific Fabs.
J Thromb Haemost. 7:1347–1355.

Lochmatter, C., Schifferli, J.A., and Martin, P.J. (2009) Schistosoma mansoni TOR is a tetraspanning orphan receptor on the parasite surface.
Parasitology. 136:487-498.

Ulinger, S. et al. (2008) The path to Platinum: The evolution of the Human Combinatorial Libraries (HuCAL ®), 19th Annual International Conference on Antibody Engineering, 2008 December 7-11. San Diego.
Presentation

Steidl, S. et al. (2008) In vitro affinity maturation of human GM-CSF antibodies by targeted CDR-diversification.
Mol Immunol. 46:135-44?

Larkin, E.A. et al. (2008) Human monoclonal antibodies against staphylococcal enterotoxin B: potential therapeutics.
Abstracts of the General Meeting of the American Society for Microbiology; 2008 June 1-5; Boston, Massachusetts.

Chernov, A.V. et al. (2008) The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
J Biol Chem. 283:17270-17278.

Rothe, C. et al. (2008) The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies.
J Mol Biol. 376:1182-1200.

Hillig, R.C. et al. (2008) Fab MOR03268 triggers absorption shift of a diagnostic dye via packaging in a solvent-shielded Fab dimer interface.
J Mol Biol. 377:206-219.

Tornetta, M. (2007) Isolation of human anti-idiotypic antibodies by phage display for clinical immune response assays.
J Immunol Methods. 328:34-44?

Barnard, G. et al. (2007) Direct detection of disease-associated prions in brain and lymphoid tissue using antibodies recognizing the extreme N terminus of PrPc.
Prion. 1:121-127.

Gustchina, E. et al. (2007) A monoclonal Fab derived from a human nonimmune phage library reveals a new epitope on gp41 and neutralizes diverse human immunodeficiency virus type 1 strains.
J Virol. 23:12946-12953.

Jarutat, T. et al. (2007) Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue.
Biol. Chem. 388: 651-658.

Kolly, R. et al. (2007) Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction.
Protein Eng Des Sel. 20:91-98.

Koch, H. et al. (2006) Direct selection of antibodies from complex libraries with the protein fragment complementation assay.
J Mol Biol. 357:427-441.

Zimmerman, T. et al. (2006) Simultaneous metal chelate affinity purification and endotoxin clearance of recombinant antibody fragments.
J Immunol Methods. 314:67-73.

Vanhoutteghem, A. and Djian, P. (2006) Basonuclins 1 and 2, whose genes share a common origin, are proteins with widely different properties and functions.
Proc Natl Acad Sci USA. 103:12423-12428.

Jarutat, T. et al. (2006) Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.
Biol Chem. 387:995-1003.

Ooe, H., Iguchi-Ariga, S.M., and Ariga, H. (2006) Establishment of specific antibodies that recognize C106-oxidized DJ-1.
Neurosci Lett. 404:166-169.

Ohara, R. et al. (2006) Antibodies for proteomic research: comparison of traditional immunization with recombinant antibody technology.
Proteomics. 6:2638-2646.

Boehncke , W.H. et al. (2005) Efficacy of the fully human monoclonal antibody MOR102 (#5) against intercellular adhesion molecule 1 in the psoriasis-severe combined immunodeficient mouse model.
J Dermatol. 153:758-766.

Poetz, O. et al. (2005) Protein microarrays for antibody profiling: specificity and affinity determination on a chip.
Proteomics. 5:2402-2411.

Haenel, C. et al. (2005) Characterization of high-affinity antibodies by electrochemiluminescence-based equilibrium titration.
Anal Biochem. 339:182-184.

Louis, J.M. et al. (2005) Characterization and HIV-1 fusion inhibitory properties of monoclonal Fabs obtained from a human non-immune phage library selected against diverse epitopes of the ectodomain of HIV-1 gp41.
J Mol Biol. 353:945-951.

Parsons, C.J. et al. (2004) Antifibrotic effects of a tissue inhibitor of metalloproteinase-1 antibody on established liver fibrosis in rats.
Hepatology. 40:1106-1115.

Röthlisberger, D., Pos, K.M., and Pluckthun, A. (2004) An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.
FEBS Lett. 564:340-348.

Sun, C. et al. (2003) Kirrel2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets.
Genomics 82:130-142.

Rauchenberger, R. et al. (2003) Human combinatorial Fab library yielding specific and functional antibodies against the human fibroblast growth factor receptor 3.
J Biol Chem. 278:38194-38205.

Cesaro-Tadic, S. et al. (2003) Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library.
Nat Biotechnol. 21:679-685.

Ewert, S. et al. (2003) Biophysical properties of human antibody variable domains.
J Mol Biol. 325:531-553.

Ewert, S., Honegger, A., and Plückthun, A. (2003) Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach.
Biochemistry. 42:1517-1528.

Frisch, C. et al. (2003) From EST to IHC: human antibody pipeline for target research.
J. Immunol. Methods 275:203-212.

Pavlickova, P. et al. (2003) Microarray of recombinant antibodies using a streptavidin sensor surface self-assembled onto a gold layer.
Biotechniques. 34:124-130.

Kretzschmar, T. and von Rüden, T. (2002) Antibody discovery: phage display.
Curr Opin Biotechnol. 13:598-602.

Blank, K. et al. (2002) Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification.
Protein Expr Purif. 24:313-322.

Nagy, Z.A. et al. (2002) Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells.
Nat Med. 8:801-807.

Krebs, B. et al. (2001) High-throughput generation and engineering of recombinant human antibodies.
J Immunol Methods. 254:67-84.

Marget, M., et al. (2000) Bypassing hybridoma technology: HLA-C reactive hman single-chain antibody fragments (scFv) derived from a synthetic phage display library (HuCAL) and their potential to discriminate HLA class I specificities.
Tissue Antigens. 56:1-9.

Hanes, J. et al. (2000) Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.
Nat Biotechnol. 18:1287-92.

Frisch, C. et al. (2000) Titration of infective and noninfective Ff filamentous bacteriophages using a monoclonal antibody against g3p.
Biotechniques. 29:26-30.

Knappik, A. et al. (2000) Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.
J Mol Biol. 296:57-86.

Plückthun, A. and Pack, P (1997) New protein engineering approaches to multivalent and bispecific antibody fragments.
Immunotechnology, 3:83-105.

Rheinnecker, M. et al. (1996) Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen.
J Immunol. 157:2989-2997.

Knappik, A. and Plückthun, A. (1995) Engineered turns of a recombinant antibody improve its in vivo folding.
Protein Eng. 8:81-90.

Virnekäs, B. et al. (1994) Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
Nucleic Acids Res. 22:5600-5607.

Pack, P. et al. (1993) Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli.
Biotechnology (NY). 11:1271-1277.