Recommended Secondary Antibodies for Applications using HuCAL® Antibodies

We offer an extensive range of secondary antibodies suitable for the detection of HuCAL antibodies used in the following applications

Western Blotting

A selection of HRP-conjugated secondary antibodies recommended for Western Blotting

Secondary Antibody Product Code
Anti-human IgG F(ab’)2:HRP STAR126P
Anti-Strep-tag® Classic:HRP MCA2489P
Anti-His-6:HRP MCA1396P
Anti-V5:HRP MCA1360P
Anti-bacterial AP:HRP AHP1108P
Anti-DYKDDDDK:HRP AHP1074P
Anti-DYKDDDDK:HRP MCA4764P
Anti-c-myc:HRP MCA2200P

ELISA

Recommended secondary antibodies ideal for use in ELISA are detailed below

Secondary Antibody Product Code
Anti-human IgG F(ab’)2:AP STAR126A
Anti-human IgG F(ab’)2:HRP STAR126P
Anti-human IgG F(ab’)2:Biotin STAR126B
Anti-Strep-tag® Classic:HRP MCA2489P
Anti-Strep-tag® Immo
(for Fab immobilization)		 MCA2488
Anti-His-6:HRP MCA1396P
Anti-V5:HRP MCA1360P
Anti-bacterial AP:HRP AHP1108P
Anti-DYKDDDDK:HRP

 

 AHP1074P
Anti-DYKDDDDK:HRP MCA4764P
Anti-c-myc:HRP MCA2200P

Immunohistochemistry (IHC)

Secondary Antibodies recommended for IHC are listed below

Secondary Antibody Product Code
Anti-His-6 tag MCA1396
Anti-AP:HRP AHP1108P
Anti-DYKDDDDK:HRP MCA4764P
Anti-V5 tag MCA1360
Anti-mouse IgG:Biotin STAR11B

Note: Anti-c-myc antibodies are not recommended for use in IHC.

Flow Cytometry

Recommended Secondary Antibodies ideal for flow cytometric analysis are detailed below

Secondary Antibody Product Code
Anti-human IgG (H+L):PE
F(ab’)2 fragment		 STAR97PE
Anti-V5:FITC MCA2894F
Anti-V5:FITC MCA2895F
Anti-DYKDDDDK:FITC MCA4764F
Anti-DYKDDDDK:PE MCA4764PE
Anti-His-6:FITC MCA1396F
Anti-His-6: PE MCA1396PE
Anti-mouse IgG (H+L):PE
F(ab’)2 fragment

 

 STAR105PE
Anti-mouse IgG:FITC
F(ab’)2 fragment		 STAR9B

Immunofluorescence

A selection of secondary antibodies recommended for immunofluorescence

Secondary Antibody Product Code
Anti-human IgG F(ab’)2:FITC STAR126F
Anti-human IgG F(ab’)2:TRITC STAR126TRC
Anti-His-6 tag:Alexa Fluor®488 MCA1396A488
Anti-His-6 tag:Alexa Fluor®647 MCA1396A647
Anti-AP AHP1108P
Anti-V5 tag MCA1360
Anti-V5 tag:Alexa Fluor® 488 MCA1360A647