An experienced group of technical advisors is available here to assist with questions regarding custom HuCAL monoclonal antibodies and their use in a wide range of applications. For help with HuCAL generated catalog antibodies and secondary reagents, contact your local AbD Serotec technical support team.
Browse our technical and application related resources below to learn more about HuCAL monoclonal antibodies and how to detect them in various assays. To learn more about the assay specific advantages of our flexible antibody formats, click here.
HuCAL antibodies can be detected in several ways, depending on the specific format of the HuCAL FAb antibody and the application of interest.
Since HuCAL monoclonal antibodies are human Fab molecules, they can be detected by antisera against human IgG F(ab’)2 preparations.
HuCAL antibodies are usually equipped with one or two epitope tags genetically fused to the
C-terminus of the antibody heavy chain. These can be detected using commercially available anti-tag antibodies, such as His-6, V5, and Strep-tag® from AbD Serotec.
In some cases bivalent Fab antibodies are generated by genetic fusion of bacterial alkaline phosphatase, which is a homo-dimer. The addition of this enzyme allows for either direct detection of alkaline phosphatase activity or indirect detection with an anti-alkaline phosphatase antibody
|Application||Protocol||Recommended Secondary Antibodies|
|Western Blotting||Protocol||HRP conjugates for Western Blotting|
|ELISA||Protocol||HRP, biotin, and AP conjugates for ELISA|
|Reagents and methods to pull down the immunocomplex|
|Immunohistochemistry||Protocol||Secondary antibodies for immunohistochemistry|
|Flow Cytometry||Protocol||Secondary antibodies for flow cytometry|
|Immunofluorescence||Protocol||Secondary antibodies for immunofluorescence|
Strep-tag® is a registered trademark of IBA, GmbH