Technical and Application Support

An experienced group of technical advisors is available here to assist with questions regarding custom HuCAL monoclonal antibodies and their use in a wide range of applications. For help with HuCAL generated catalog antibodies and secondary reagents, contact your local AbD Serotec technical support team.

Browse our technical and application related resources below to learn more about HuCAL monoclonal antibodies and how to detect them in various assays. To learn more about the assay specific advantages of our flexible antibody formats, click here.


Technical Resources

  1. HuCAL Antibodies Technical Manual with a technology review, examples, and protocols
  2. Recommended Fab antibody formats by application
  3. Fab antibody formats and epitope tag combinations
  4. Epitope tag sequences and homodimerization domains
  5. Reagents to Support HuCAL® Assay Development
  6. HuCAL reference publications

Detection of HuCAL Monoclonal Antibodies

HuCAL antibodies can be detected in several ways, depending on the specific format of the HuCAL FAb antibody and the application of interest.

  • Detection of the Fab scaffold

Since HuCAL monoclonal antibodies are human Fab molecules, they can be detected by antisera against human IgG F(ab’)2 preparations.

  • Detection of embedded epitope tags

HuCAL antibodies are usually equipped with one or two epitope tags genetically fused to the
C-terminus of the antibody heavy chain. These can be detected using commercially available anti-tag antibodies, such as His-6, V5, and Strep-tag® from AbD Serotec.

  • Detection of alkaline phosphatase fusion partner

In some cases bivalent Fab antibodies are generated by genetic fusion of bacterial alkaline phosphatase, which is a homo-dimer. The addition of this enzyme allows for either direct detection of alkaline phosphatase activity or indirect detection with an anti-alkaline phosphatase antibody


HuCAL Protocols and Recommended Secondary Antibodies

Application Protocol Recommended Secondary Antibodies
Western Blotting Protocol HRP conjugates for Western Blotting
ELISA Protocol HRP, biotin, and AP conjugates for ELISA
Immunoprecipitation Protocol

 

Reagents and methods to pull down the immunocomplex
Immunohistochemistry Protocol Secondary antibodies for immunohistochemistry
Flow Cytometry Protocol Secondary antibodies for flow cytometry
Immunofluorescence Protocol Secondary antibodies for immunofluorescence
Bead-based Assays Protocol  

Strep-tag® is a registered trademark of IBA, GmbH

Anti-idiotypic antibodies

Article - Using monoclonal antibodies to improve assays for antibody drug development

Article: Using Human Monoclonal Antibodies to Improve Assays for Antibody Drug Development...

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