Product Images

  • c-myc tagged protein detected with Mouse anti c-myc:Alk Phos (MCA2200A)
  • Western blot analysis of <a rel="nofollow" href='http://hek293.com/'target="_blank">HEK293</a> embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody (MCA2200) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence

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List Price Quantity
MCA2200A647
TBC

c-Myc Antibody

  • Product Code
    MCA2200A647
  • Description
    Mouse anti c-Myc:Alexa Fluor® 647
  • Product Type
    Monoclonal Antibody
  • Clone
    9E10
  • Isotype
    IgG1
  • Format
    Alexa Fluor® 647
  • Applications
    F *
  • Pack Size
    100 Tests/1ml
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

Specificity

Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines (Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999).

Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preservative Stabilisers

0.09%Sodium Azide
1%Bovine Serum Albumin

Immunogen

Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Buffer Solution

Phosphate buffered saline

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.
  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
    MCA2200A488
    Alk. Phos.MCA2200A0.1 mgC, E, P, WB*
    MCA2200A
    BiotinMCA2200B0.1 mgC, E, F *, P, WB*
    MCA2200B
    DyLight®549MCA2200D549GA0.1 mgF, IF
    MCA2200D549GA
    FITCMCA2200F0.1 mgF *
    MCA2200F
    HRPMCA2200P0.1 mgC, E, P, WB*
    MCA2200P
    PurifiedMCA2200GA0.1 mgC, E, F *, P, WB*
    MCA2200GA
    PurifiedMCA22001 mgC, E, F *, P, WB*
    MCA2200
    PurifiedMCA2200G2 mgC, E, F *, P, WB*
    MCA2200G
    PurifiedMCA2200T20 µgC, E, F *, P, WB*
    MCA2200T
    RPEMCA2200PE100 TestsF *
    MCA2200PE

Recommended Negative Isotype Control

FormatProduct CodePack SizeApplicationsList PriceQuantity
Alexa Fluor® 647MCA928A647100 Tests/1mlF
MCA928A647

More Reagents With This Specificity

Application

Application NameYesNoNot DeterminedSuggested Dilution
Flow Cytometry(1)Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.

(1)
Membrane permeabilisation is required for this application. Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.

Flow Cytometry

Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

References

1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product.
Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Anticancer Res. 7: 1299 -1304.
3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.
Microb. Cell Fact. 9: 74-86.
4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.
Biochem J. 404: 23-9.
5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.
J Cell Biol. 187: 959-66.
6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
Protein Eng. 14: 803-6.
7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
Proteins. 73: 552-65.
8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.
J Immunol. 184: 6359-66.
9. Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.
J Biol Chem. 285: 26878-88.
10. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
Blood. 106: 2936-43.
11. Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.
Mol Biol Cell. 21: 2996-3006.

Further Reading

1. Nesbit, C. et al. (1999) MYC oncogenes and human neoplastic disease.
Oncogene. 18: 3004-16.
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