Product Images

  • c-myc tagged protein detected with Mouse anti c-myc:Alk Phos (MCA2200A)
  • Western blot analysis of <a rel="nofollow" href='http://hek293.com/'target="_blank">HEK293</a> embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody (MCA2200) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence

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MCA2200G
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c-Myc Antibody

  • Product Code
    MCA2200G
  • Description
    Mouse anti c-Myc
  • Product Type
    Monoclonal Antibody
  • Clone
    9E10
  • Isotype
    IgG1
  • Format
    Purified
  • Applications
    C, E, F *, P, WB*
  • Pack Size
    2 mg
Product Summary
Additional Formats
Related Reagents
More Images
Application Notes
References
More Information

c-Myc Antibody Product Summary

Specificity

Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines (Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999).

Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preservative Stabilisers

0.09%Sodium Azide

Immunogen

Synthetic peptide sequence corresponding to the C-terminal region (residues 408-439) of human c-myc conjugated to keyhole limpet hemocyanin.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Buffer Solution

Phosphate buffered saline

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.

Storage

Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

c-Myc Antibody Additional Formats

  • FormatProduct CodePack SizeApplicationsList PriceQuantity
    Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
    MCA2200A488
    Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
    MCA2200A647
    Alk. Phos.MCA2200A0.1 mgC, E, P, WB*
    MCA2200A
    BiotinMCA2200B0.1 mgC, E, F *, P, WB*
    MCA2200B
    DyLight®549MCA2200D549GA0.1 mgF, IF
    MCA2200D549GA
    FITCMCA2200F0.1 mgF *
    MCA2200F
    HRPMCA2200P0.1 mgC, E, P, WB*
    MCA2200P
    PurifiedMCA2200GA0.1 mgC, E, F *, P
    MCA2200GA
    PurifiedMCA22001 mgC, E, F *, P, WB*
    MCA2200
    PurifiedMCA2200T20 µgC, F *, P
    MCA2200T
    RPEMCA2200PE100 TestsF *
    MCA2200PE

c-Myc Antibody Related Reagents

Recommended Secondary Antibody

FormatProduct CodePack SizeApplicationsList PriceQuantity
Alk. Phos.STAR117A0.5 mgE, WB
STAR117A
Alk. Phos.STAR87A1 mgC, E, WB
STAR87A
DyLight®488STAR117D488GA0.1 mgF, IF
STAR117D488GA
DyLight®549STAR117D549GA0.1 mgF, IF
STAR117D549GA
DyLight®649STAR117D649GA0.1 mgF, IF
STAR117D649GA
DyLight®800STAR117D800GA0.1 mgF, IF, WB
STAR117D800GA
DyLight®800STAR8D800GA0.1 mgF, IF, WB
STAR8D800GA
FITCSTAR117F0.5 mgF
STAR117F
FITCSTAR700.5 mgF
STAR70
FITCSTAR120F1 mgC, F
STAR120F
FITCSTAR9B1 mgF
STAR9B
HRPHCA036P0.1 mgE
HCA036P
HRPSTAR117P0.5 mgE, WB
STAR117P
HRPSTAR770.5 mgC, E, P
STAR77
HRPSTAR120P1 mgE, WB
STAR120P
HRPSTAR13B1 mgC, E, P, RE, WB
STAR13B
HRPSTAR87P1 mgE
STAR87P
RPESTAR12A100 TestsF
STAR12A
RPESTAR76100 TestsF
STAR76

Recommended Negative Isotype Control

FormatProduct CodePack SizeApplicationsList PriceQuantity
PurifiedMCA928100 TestsC, F
MCA928

More Reagents With This Specificity

c-Myc Antibody More Images

c-Myc Antibody Application Notes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.abdserotec.com/protocols.
Application NameYesNoNot DeterminedSuggested Dilution
ELISA1/100 - 1/500
Flow Cytometry(1)1/10 - 1/50
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(2)1/100 - 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
(1)
Membrane permeabilisation is required for this application. Serotec recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
(2)
9E10 recognizes c-myc under non-reducing conditions

Flow Cytometry

Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Immunohistology

This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

c-Myc Antibody References

References

1. Evan, G.I. et al. (1985) Isolation of monoclonal antibodies specific for human c-myc. proto-oncogene product.
Mol. Cell. Biol. 5: 3610 -3616.
2. Spandidos, D. A. et al. (1987) Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Anticancer Res. 7: 1299 -1304.
3. Borodina, I. et al. (2010) Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae.
Microb. Cell Fact. 9: 74-86.
4. Groeger, G. et al. (2007) Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction.
Biochem J. 404: 23-9.
5. Head, B. et al. (2009) Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells.
J Cell Biol. 187: 959-66.
6. Hilpert, K. et al. (2001) Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
Protein Eng. 14: 803-6.
7. Krauss, N. et al. (2008) The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops.
Proteins. 73: 552-65.
8. Gray, P. et al. (2010) Identification of a novel human MD-2 splice variant that negatively regulates Lipopolysaccharide-induced TLR4 signaling.
J Immunol. 184: 6359-66.
9. Duriseti, S. et al. (2010) Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration.
J Biol Chem. 285: 26878-88.
10. Tan, P.H. et al. (2005) Creation of tolerogenic human dendritic cells via intracellular CTLA4: a novel strategy with potential in clinical immunosuppression.
Blood. 106: 2936-43.
11. Wallace, S.W. et al. (2010) Cdc42 regulates apical junction formation in human bronchial epithelial cells through PAK4 and Par6B.
Mol Biol Cell. 21: 2996-3006.

Further Reading

1. Nesbit, C. et al. (1999) MYC oncogenes and human neoplastic disease.
Oncogene. 18: 3004-16.

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