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C3a antibody | H13

Mouse anti Human C3a

Product Type
Monoclonal Antibody
Clone
H13
Isotype
IgG2b
Specificity
C3a

Product Code Applications Pack Size List Price Your Price Qty
0200-0615
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 50 µg loader
List Price Your Price
loader

Mouse anti Human C3a antibody, clone H13 recognizes human C3a anaphylatoxin, a cleavage product of human complement component C3. C3a increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Mouse anti Human C3a antibody, clone H13 recognizes a C3a-determinant also present on native C3. Mouse anti Human C3a antibody, clone H13 allows discrimination of ubiquitous or non-specifically bound native C3 or C3a from biologically active C3b fragment, neither C3b or C5 components are recognized by Mouse anti Human C3a antibody, clone H13 (Burger et al. 1987).

Mouse anti Human C3a antibody, clone H13 is reported to block C3a functional activity (Hartmann et al. 1997).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Guinea Pig
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide, 0.5% Bovine Serum Albumin.
Immunogen
Human complement component C3.
Approx. Protein Concentrations
IgG concentration 50 ug/ml after reconstitution.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the non-secreting P3-X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen 1/10
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Source Reference

  1. Burger, R. et al. (1987) Functional analysis and quantification of the complement C3 derived anaphylatoxin C3a with a monoclonal antibody.
    Clin Exp Immunol. 68 (3): 703-11.

References for C3a antibody

  1. Burger, R. et al. (1988) The C terminus of the anaphylatoxin C3a generated upon complement activation represents a neoantigenic determinant with diagnostic potential.
    J Immunol. 141 (2): 553-8.
  2. Hartmann, K. et al. (1997) C3a and C5a stimulate chemotaxis of human mast cells.
    Blood. 89 (8): 2863-70.
  3. Möller, T. et al. (1997) Mechanisms of C5a and C3a complement fragment-induced [Ca2+]i signaling in mouse microglia.
    J Neurosci. 17 (2): 615-24.

Synonyms
Complement Component 3a
RRID
AB_2195325
UniProt
P01024
Entrez Gene
C3
GO Terms
GO:0005102 receptor binding
GO:0004866 endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0010575 positive regulation vascular endothelial growth factor production

0200-0615

162221 164753

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